Tissue Culture Questions . . .

Hey,

Clutcher? Culture. Nucular? Nuclear . . .

I was wondering how many in this forum have had experience with any of the readily available home tissue culture setups? I recall doing a bit of TC in college (soybeans and a few other things I'd rather not disclose) but that was a serious environment with laminar flow hoods, autoclaves, and regretable squirt bottle fights with carbon tet. We were successful with all of the assigned experiments but it came as more of a surprise in that particular class for too many reasons . . .

If anyone could offer some advice with this, it would be greatly appreciated . . .

Carol Stiff's kitchen culture kit is the best start-up kit around.

It's what I started with and it's been a great tool for me.

Hope this helps,
Nate

Bella,
I have a freind in the fairfield who is an avid TCist. I'll check with him to see if it is okay that you contact him..
LMO

flytraplady5 said:

Bella,
I have a freind in the fairfield who is an avid TCist. I'll check with him to see if it is okay that you contact him..
LMO

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Thanks. Would that be David Conner? I had a chance to briefly speak to him during the BACPS meeting at California Carnivores.

I would love to get into this too. Dec can't come soon enough so I have time to do some reading.

JB_OrchidGuy said:

I would love to get into this too. Dec can't come soon enough so I have time to do some reading.

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Yeah, it's been something that has always interested me -- ever since I obtained a flask of Cephalotus from the former World Insectivorous Plants as a kid in the early 1980s, that and the mad scientist phase(s) previously mentioned in college. Some of the chemicals used in TC -- colchicine, for example -- we've used in connection with oyster culture (just as in plants, to induce polyploidy); and we obtained a number of our supplies in years past, especially while overseas, from tissue culture labs and what they were working on was always really interesting. I recently had a chance to talk with someone a few weeks ago -- however briefly -- who was culturing Dionaea B52 and other cultivars quite successfully, but there were too many people and not enough time to ask any informed questions . . .