As part of my Horticulture education at Penn State University, I will be performing a study of pinguicula species and hybrid propagation via traditional and tissue culture methods on varying substrate(s) and substrate composition respectively. I plan the study as follows:
(Look at my growlist to see the pings to be used in the study, I hope to use all, however this might not prove to be possible.)
Leaf pullings will be placed in small propagation trays (actually chinese takeout containers but boy do they work well) on;
Long fibered spaghnum
possibly a combination of the above media though that is to be determined
Any suggestions for other media to test?
I will grow the parent pings and leaf pullings in a greenhouse under MH lights with cappilary matting for irrigation. (DW of course)
Concurrently, leaves of all representative pings will be placed into tissue culture to compare efficacy of propagation as well as trueness to type(DNA analysis on propagules and parents to be performed.) Media recipe will be the same as reported by Adams, Koenigsberg, and Lanhans 1979, though I won't be doing the high salt treatment they performed since that will just mean dead pings as they showed.
I hope to see that TC produces the highest number of plantlets, but also has a higher incidence of mutation. From there I hope to determine the optimal media for "traditional" propagation.
I know that many members have their own methods for propagation and cultivation so if you would like to share methods, ideas, stories, conjectures or otherwise, I will be sure to acknowledge both your contribution as well as terraforums for providing this message board.