Hey guys,
Been reading up on Tissue Culture so on but I didn't wanna pay a lot to give it a whirl so while I was heatng up my TV dinner tonight I put this little puppy together:
Made with a 66 quart clear Sterilite storage box (with white lid) from target
(no that's not anything illegal on the table it's only catnip- the cats run from the smell of new plastic and silicone
)
The "arm hole guards" or whatever you calll them are made from 4" diameter Closet Flange(s) from Home Depot siliconed in place
all sealed up and Ready to Rock! (other hand taking the pic)
Here's how I did it if you happen to be interested in sterile techniques but wanna save some $$:
1) trace the outer diameter of the closet flanges on the lid with a sharpie
2) using a drill trace the lines drilling every 1/2" or so
3) put the drill aside and grab a heavy duty utility knife and open a vein, no, be very careful and trace the knife from those drilled holes one to the next like connect the dots- here you will have two nice holes
4) test fit the closet flanges in the holes, put box upright (top facing up)
5) put silicone on the back of the front of the flange, place in holes, give a slight pressure and twist to secure in place and let dry
6) remove lid and apply silicone around the inside edge where the flanges emerge from the lid, this will super seal the gaps around the arm holes to make sure no air is getting in
This isn't my invention, I saw it somewhere online and made notes. It took me only 15 minutes to do the bulk of the project. compare that with buying a sterile transfer hood (even without a HEPA flow) and you're looking at way more than this which cost me all of about $20 :
Box $8
2 Plastic Closet Flanges $8
Silicone $3
Of course you could also cut another hole and attach a small $20 HEPA filter unit from Wal Mart to give you everything for about $40 if it's really needed.
I hope someone finds a use for this info!
Been reading up on Tissue Culture so on but I didn't wanna pay a lot to give it a whirl so while I was heatng up my TV dinner tonight I put this little puppy together:
Made with a 66 quart clear Sterilite storage box (with white lid) from target
(no that's not anything illegal on the table it's only catnip- the cats run from the smell of new plastic and silicone
)
The "arm hole guards" or whatever you calll them are made from 4" diameter Closet Flange(s) from Home Depot siliconed in place
all sealed up and Ready to Rock! (other hand taking the pic)
Here's how I did it if you happen to be interested in sterile techniques but wanna save some $$:
1) trace the outer diameter of the closet flanges on the lid with a sharpie
2) using a drill trace the lines drilling every 1/2" or so
3) put the drill aside and grab a heavy duty utility knife and open a vein, no, be very careful and trace the knife from those drilled holes one to the next like connect the dots- here you will have two nice holes
4) test fit the closet flanges in the holes, put box upright (top facing up)
5) put silicone on the back of the front of the flange, place in holes, give a slight pressure and twist to secure in place and let dry
6) remove lid and apply silicone around the inside edge where the flanges emerge from the lid, this will super seal the gaps around the arm holes to make sure no air is getting in
This isn't my invention, I saw it somewhere online and made notes. It took me only 15 minutes to do the bulk of the project. compare that with buying a sterile transfer hood (even without a HEPA flow) and you're looking at way more than this which cost me all of about $20 :
Box $8
2 Plastic Closet Flanges $8
Silicone $3
Of course you could also cut another hole and attach a small $20 HEPA filter unit from Wal Mart to give you everything for about $40 if it's really needed.
I hope someone finds a use for this info!
