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Thread: DIY Sterile Transfer chamber in 15 mins!

  1. #1
    swords's Avatar
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    DIY Sterile Transfer chamber in 15 mins!

    Hey guys,

    Been reading up on Tissue Culture so on but I didn't wanna pay a lot to give it a whirl so while I was heatng up my TV dinner tonight I put this little puppy together:



    Made with a 66 quart clear Sterilite storage box (with white lid) from target
    (no that's not anything illegal on the table it's only catnip- the cats run from the smell of new plastic and silicone )



    The "arm hole guards" or whatever you calll them are made from 4" diameter Closet Flange(s) from Home Depot siliconed in place


    all sealed up and Ready to Rock! (other hand taking the pic)

    Here's how I did it if you happen to be interested in sterile techniques but wanna save some $$:

    1) trace the outer diameter of the closet flanges on the lid with a sharpie
    2) using a drill trace the lines drilling every 1/2" or so
    3) put the drill aside and grab a heavy duty utility knife and open a vein, no, be very careful and trace the knife from those drilled holes one to the next like connect the dots- here you will have two nice holes
    4) test fit the closet flanges in the holes, put box upright (top facing up)
    5) put silicone on the back of the front of the flange, place in holes, give a slight pressure and twist to secure in place and let dry
    6) remove lid and apply silicone around the inside edge where the flanges emerge from the lid, this will super seal the gaps around the arm holes to make sure no air is getting in

    This isn't my invention, I saw it somewhere online and made notes. It took me only 15 minutes to do the bulk of the project. compare that with buying a sterile transfer hood (even without a HEPA flow) and you're looking at way more than this which cost me all of about $20 :
    Box $8
    2 Plastic Closet Flanges $8
    Silicone $3
    Of course you could also cut another hole and attach a small $20 HEPA filter unit from Wal Mart to give you everything for about $40 if it's really needed.

    I hope someone finds a use for this info!

  2. #2
    rco911's Avatar
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    DUDE, THAT IS STRAIGHT ILL. WOW! Thanks for sharing!!!

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    Two flies one pitcher. Minus the crap eating. obregon562's Avatar
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    swiggity sweet!
    "It's easy to rip on cops, when you aren't the one needing saving"

    My Growlist

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    OMG h8 pings MrFlyTrap2's Avatar
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    Looks good! Let us know how it works!

    I'm curious if you're gonna have problems with jars falling over. The biggest problem I have with working in home made hoods and such is that space gets short quickly, and tools are awkard due to size.

    Does your container make a good seal, or are you gonna tape it up too? Looks like the ones I pick up from walmart, they never grip right for me and the cover is always popping off.

    -Nate
    My Grow List

    "It is only by studying nature that can we ever hope to defeat it."

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    swords's Avatar
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    Yes, most boxes are angled but this one is nice and almost perfectly square - I shopped around to find one like it - Target is the place!
    I could use plastic tape or something however, the lid sticks on really well - I can carry it aroudn with my arms through the holes. It has some tabs on either end's handle that "snap" to hold it in place. To ease it open just lift a side handle away from the side and the tabs let go - it will kind of swing open like it's on a hinge from the other tabs on the oposite side, as in pic #2. Definately pays to look around to find a box you like.

  6. #6
    OMG h8 pings MrFlyTrap2's Avatar
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    Ah! There you go, we have a mini target here so they don't have that great of a selection.

    Keep us updated!

    -Nate
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    "It is only by studying nature that can we ever hope to defeat it."

  7. #7
    heatherfeather knows the weather! lil hokie's Avatar
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    Wow, that looks awesome.

  8. #8
    rco911's Avatar
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    LOL - gotta love that warning sign too - remember, no lil kids in the chamber, k? heh

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