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Thread: Western 2.5 TC media for CP's ?

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    Illinois droseraguy's Avatar
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    Western 2.5 TC media for CP's ?

    Has anyone got any experience using Western 2.5 media for tissue culturing CP's ? I mixed up a batch and forgot to add the coconut liquid for my paph. seed. No good for them now. I'd hate to throw it out.
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    OMG h8 pings MrFlyTrap2's Avatar
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    I'm not sure how you mixed your solution, but if you used agar, you can heat it up, remelt it and add in the part you forgot. The tricky part will be keeping the solution hot while collecting and redistributing it.
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    Can you just reheat the media and add the ingrediant you forgot
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    D_muscipula's Avatar
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    what is western 2.5 media?
    IU know what tc is and have read about it and would like to do it someday, hey did you know agar is like a gelling agent used in Asian foods. If you go to the supermarket to get it make sure it isn't chocolate or vanilla you want the plainest stuff you can find.
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    make sure it isn't chocolate or vanilla you want the plainest stuff you can find
    Yeah I always get the low carb summer strawberry.
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    Illinois droseraguy's Avatar
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    Interesting, never even thought about reworking it. Thanks guys I'll give it a shot.
    Only two defining forces have ever offered to die for you, Jesus Christ and The American G. I. One died for your soul, the other for your freedom.
    Romans 10: 9-13
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