I've been playing around with tissue culture for the last couple months. It's an interesting hobby, I really enjoy it. So far I have had little success with explants. Either I get contamination or I kill the tissue. I haven't found the happy medium between the two yet.
All these are grown from seed on 1/2 MS, no hormones.
For fun, Siam Ruby banana meristem (1/2 MS, 5mL/BAP per liter) This media didn't gel properly due to the hormones. When using hormones, use more agar!
My setup, I've been at it for awhile. These are from all different dates. Not a whole lot is happening yet.
Anyone whom is interested in TC, read. Read any and all information that you can find. Some of the information will be good, some won't. Due to bad information, the first two batches of media that I made failed completely because it didn't gel correctly. Some things will work well for you while others will not. Also, start with seed. There's nothing more disappointing than doing all the work, waiting a week to three and start to see contamination.
The next experiment in getting explants to work is starting the leaf cuttings in distilled water (Pinguicula and Drosera in particular) and using the material that sprouts off of it. In theory this should be cleaner than using a leaf right off the plant. Only time will tell. I have some plantlets forming from roots in bags with moist Sphagnum. I'm in the process of trying this.