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Thread: I am back with some TC pics:-)

  1. #1
    klasac's Avatar
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    I am back with some TC pics:-)

    Hi, I took some pics of some TC jars:-)

    drosera x tokaiensis


    drosera auriculata seedlings


    drosera ascendens germination


    drosera intermedia 'Algonquin park, Ontario, Canada' on semi-liquid medium


    drosera montana


    drosera rotundifolia seedlings


    drosera spatulata 'green'


    drosera burmannii


    part of my setup

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    instigator thez_yo's Avatar
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    Doesn't it get too hot in those jars, sealed and so close to the lights?

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    BigBella's Avatar
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    Very cool . . .

    It's great to see how far tissue culture has come from strictly laboratory settings -- and, finally, to consumers and hobbyists. When we were experimenting with TC in college, it was a bit on the exotic side and still quite expensive . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    dustin's Avatar
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    nice i hope to start TC'ing soon, have any tips? media prep?

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    ermahgerd petmantis's Avatar
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    Very, very cool
    <Heli> How are you guys losing your hamatas?
    <Brokken> Heli: The hamburglar.

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    Jag's Avatar
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    Klasac: Maybe you can do a thread where you explain your successful process in doing tissue culture and what products to use?

    If you don't mind ofcourse.
    Growlist

    Jesse

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    Illinois droseraguy's Avatar
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    Yes please, I'm really interested in your seed/tissue sterilization protocol.
    Only two defining forces have ever offered to die for you, Jesus Christ and The American G. I. One died for your soul, the other for your freedom.
    Romans 10: 9-13
    Growlist http://www.cpukforum.com/forum/index...04&#entry90304

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    klasac's Avatar
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    Hi, no, it does not get too hot in the jars, since they are under white cool fluorescent lights.
    Here are some pics of my low-cost setup:
    My "laminar flow hood", which you can call it only with exaggeration:-) Basically an old cupboard with some walls replaced with glass and flexible rubber shields on the bottom (cut off- exercise book covers;-))


    Inside, some beakers with sterilant, sterile water and alcohol, tweezers and spray.

    The jars and medium are sterilised in pressure cooker and solidified when cooled


    Then I get all the things needed assembled (seeds, tissue,parafilm,.....)


    And after hours of tedious work inside, I get my plants ready to grow and seeds ready to germinate (if no contamination or sudden death occurs)

    Later they look like this:


    ...and few months later of doing nothing they are ready to be replated or deflasked. (here, picture of drosera montana var. glabrata and drosera peltata from jar during deflasking process)




    ...and some hardened plants:


    just wanted to show that it can be done at home with some degree of success if there is a will:-)

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