User Tag List

Informational! Informational!:  0
Likes Likes:  0
Page 1 of 4 1234 LastLast
Results 1 to 8 of 26

Thread: Writing my Thesis

  1. #1

    Join Date
    Jan 2009
    Posts
    1,672
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)

    Writing my Thesis

    I will soon be starting to write a proposal for a thesis for my Master's degree and have played with a couple ideas in the botany/microbiology field. I have one that sounds the appealing and I think could be potentially very interesting to the community here all the while fulfilling my requirements: trichoderma and Sarracenia.

    I'm starting with a broad idea and would like to narrow it down to a single strain of trichob(T22?) and down to a single species of Sarr (purp?) so that I am able to make more conclusive results.
    I know a few of you here have dealt with writing a thesis so I would be very appreciative of any do's and don'ts like DON'T wait until last minute etc...etc... Also if anyone knows how to or where I could get access to a large amount of Trich and a large amount of identical plants this would save some headache. Other topic ideas are also welcome. Basically if you have an input please post

    This semester will consist of just writing a proposal so any data collection won't begin until next year at the earliest but I figured I'd get a step ahead of the game and prep as best I can. Once all is done I'll be more than happy to share my results as well as my thesis for those of you interested.

    Thanks to all in advance

  2. #2
    Let's positive thinking! seedjar's Avatar
    Join Date
    Dec 2004
    Location
    Olympia, Washington
    Posts
    4,064
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Well, I don't have a lot of experience with a formal master's thesis, but I've written a number of papers that my professors/teachers referred to as the like, so I can at least appreciate the scale of your undertaking. Yes, don't stall on it. Also, pick an easily quantifiable question. The question itself doesn't have to be easy, just make sure that you won't have to jump through a lot of hoops to relate your experiment to your hypothesis and conclusion.
    I think S. purpurea is a great test subject for the matter - I believe that some of the big industrial nurseries tissue culture it, so you could find clones that way. If you're familiar with the process of striking purps from leaf cuttings, that would make for even more subjects from the same clonal stock.
    Take a look at some literature on the matter and see what other people are investigating with Trichoderma. I know there's a lot of contention among horticultural inoculant brands as to the best strains, best mixes, best delivery methods, etc. You may want to have Trichoderma strains be your control variable - investigate the efficacy of different strains or mixes of strains. Keep it simple, but I think one mycorrhizae and one host is a little bit too simple. Unless, I guess, you were doing some sort of microscope study of growth habits or something, but I'm not very experienced with microbiology so I'm drawing a blank.
    Anyways, take your time to come up with something you're keen on. But keep in mind that by the time you're finishing your paper, you'll be pulling all nighters, skipping meals, and neglecting everyday hygiene for the sake of milking every remaining second before your deadline. For the sake of your sanity, try to channel some of that impending fervor into your research now, so that you're totally prepared and don't have to backtrack when it comes time to do your labs.
    Best luck,
    ~Joe
    o//~ Livin' like a bug ain't easy / My old clothes don't seem to fit me /
    I got little tiny bug feet / I don't really know what bugs eat /
    Don't want no one steppin' on me / Now I'm sympathizin' with fleas /
    Livin' like a bug ain't easy / Livin' like a bug ain't easy... o//~

  3. #3
    Av8tor1's Avatar
    Join Date
    Jan 2007
    Posts
    4,811
    Mentioned
    6 Post(s)
    Tagged
    0 Thread(s)
    i cant help with T22 but i can probably help with certain proprietary strain of T. atroviride (sp?)
    Depends on the definition of "a large amount" Yann

    You might also approach TF member "true424" (Kelly @ Ampac Biotech)
    I think she may have helped provide some similar resource materials to Barry Rice for a research project he is involved with
    :P

  4. #4
    Not Growing Up! GrowinOld's Avatar
    Join Date
    Jun 2008
    Location
    ARTificial Bog in da' Middle of da' USA
    Posts
    932
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)

    Thesis

    Okay,
    "Don't wait till the last minute!"
    There, that was easy!

    More seriously, indeed it sounds like you have a large, long term experiment to do. (The thesis may end up being about how to deal with experimental failure when all the subjects/samples die after a years worth of work!) BUT, looking on the bright side and staying positive, I had a couple thoughts when reading your request...

    I am concerned about narrowing down the type of Tricho to use (T22?) without knowing if it is a wise choice compared to another. Indeed chosing one type of Tricho is good, but which one? It could be the one type that doesn't develop a symbiotic relationship with Sar.Purp's!
    So to me, I would also consider a couple side experiments that involve some other Tricho types, just to see if anything is noticed. (I also wonder if variations in environmental conditions could affect the results?... Like the Ph of the growing media for example?)

    As for tricho, I have often gotten some that are mixes of many varieties, but the only person I know currently who works with large amounts of a single type of Trichoderma (atroviride), I would contact Kelly (TF: true424). I am sure she would be glad to give you more info.

    As for plants, you might consider buying a cloned/TC plant from a TC lab/grower, as these should all be as identical as you can expect. Either that, or buy a huge plant and start making cuttings/divisions of it!

    Good luck... and
    Keep us updated!

    Paul

    ---------- Post added at 11:05 AM ---------- Previous post was at 11:04 AM ----------

    Aww,
    AV!
    You just beat me in by a fraction of a second!
    Experience is the best teacher. At least it used to be.
    But then, common sense isn't so common anymore, is it.


    http://www.terraforums.com/forums/sh...d.php?t=113866

  5. #5
    Av8tor1's Avatar
    Join Date
    Jan 2007
    Posts
    4,811
    Mentioned
    6 Post(s)
    Tagged
    0 Thread(s)
    hehehehe Paul

    i just read your mind is all

  6. #6

    Join Date
    Jan 2009
    Posts
    1,672
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Thanks all, your inputs are already helping me out immensely. I think testing multiple Tricho strains would be the ideal way to go based on the suggestions. I will contact Kelly and see if we can strike some sort of deal. I still need to meet with my mentoring professor but I wanted to show up with at least some sort of plan.
    As for propagating plants from divisions: I think that would be the best route to ensure identical subjects; unfortunately I would be afraid that these divisions would not have sufficient time to develop an extensive root system by the time the trich was to be added. I would also be weary of my results since Trich is an opportunistic fungi and the subjects would have just undergone "stress". I think the best route sounds like contacting a TC lab such as the ones that supply LOWES with the death cubes and nurse the plants back to health in time for trials.

    Thanks all! Keep em coming!

  7. #7
    Carnivorous plant enthusiast vraev's Avatar
    Join Date
    Aug 2006
    Location
    Ontario, Canada
    Posts
    2,806
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Also keep in mind that in order to do this experiment properly, you might have to run it for atleast a few months to an year. I would suggest to have some backup plants as well...I mean these ones are non carnivorous. There are quite a few variables with CPs to control, so perhaps a backup with regular tomato plants or somehting might be a nice fallback strategy if things don't go as planned.

  8. #8
    Av8tor1's Avatar
    Join Date
    Jan 2007
    Posts
    4,811
    Mentioned
    6 Post(s)
    Tagged
    0 Thread(s)
    another issue would be contamination by insect transfer.... an insect can very easily carry spores and cross contaminate your groups

    you will have to have some way of verifying the integrity of the groups, both for trichoderma growth and no trichoderma growth.
    I have some research papers that go into methods for this, look through my research folder Yann.

    Butch

Page 1 of 4 1234 LastLast

Similar Threads

  1. Thesis done!
    By Mokele in forum General Discussions
    Replies: 11
    Last Post: 05-28-2008, 01:43 PM
  2. Replies: 16
    Last Post: 01-09-2008, 02:07 PM
  3. Writing software (again)
    By swords in forum General Discussions
    Replies: 1
    Last Post: 11-19-2007, 02:22 AM
  4. Writing a letter....
    By RyanG in forum General Discussions
    Replies: 4
    Last Post: 03-10-2007, 01:05 PM
  5. Can someone help me with writing scripts and stuff
    By superimposedhope in forum General Discussions
    Replies: 5
    Last Post: 02-26-2005, 04:37 PM

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •