Great work! I'd be interested in seeing your work area also. What do you do to control airflow & mitigate contamination?Wow!!! Very nice cp tc varieties you got going on. What is your sterilization procedure? I would love pics of your tc area
This time around I just used MS with PPM to sterilize everything. Now, I got a few contams but so far its working out okay. I do not have a LFH and I do all my TCing in my living room.What is your sterilization procedure? I would love pics of your tc area
The heli seeds got contaminated. I even tried taking them out and resterilizing them without success. Its part of the reason I stopped using bleach and peroxide to sterilize explants.i do hope that you're able to induce a callous with the heliamphora
Make sure theres no fans on and the air is still as possible, lysol and PPM.What do you do to control airflow & mitigate contamination?
It died. Didnt get any contams just never grew and finally turned black. Waiting on my cephs to start putting out winter leaves then i'll be trying again.What happened with the ceph explant?
The lysol is for cleaning surfaces etc. I use 1/3 MS and a PPM solution to sterilize the explants. Last run I used 2ml PPM in 4 oz of 1/3 MS. Not too many contams...actually none on the heli seed and ceph explants which were the last things I plated. Even have a callus forming on a ceph leaf finally. 1ml BAP per liter of media is what I used to initiate the callus.What was your sterilization procedure (lysol and PPM)?
Not for me but they divide like crazy.Do sarr seeds grow faster when grown this way?