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Thread: Small tissue culturing success

  1. #25
    sarracenia lover dionae's Avatar
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    Smile

    Thank you sig. I've had plenty contams...I just dont post pics of them. Thanks for the kind words and thanks for stopping by!

    ---------- Post added at 06:14 AM ---------- Previous post was at 06:02 AM ----------

    Hello everyone! Not much going on with my TCing lately but I have a few things to share pics of. Dentes are getting huge. Dews are ready for a replate. Just flasked up some heliamphora seed.

    H. heterodoxa x minor



    Dente





    Random VFTs including a mutant







    D aliciae



    D capensis all red


  2. #26
    sarracenia lover dionae's Avatar
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    Smile New Flasks

    Thought i'd share a few pics of some recently flasked and replated stuff.

    VFT seedling germinated in vitro. Freshly replated onto new media.



    Dente explants separated and put onto new media





    Typical VFT explant



    S flava rubricorpora x S flava Ornata



    N kohola x rokko x



    S purpurea



    S oreo


  3. #27
    Rob's Avatar
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    Wow!!! Very nice cp tc varieties you got going on. What is your sterilization procedure? I would love pics of your tc area

  4. #28
    i dont do pots. amphirion's Avatar
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    very nice TC work sir....i do hope that you're able to induce a callous with the heliamphora.....that would be amazing. maybe with the nepenthes as well!
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
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  5. #29
    RL7836's Avatar
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    Quote Originally Posted by Rob View Post
    Wow!!! Very nice cp tc varieties you got going on. What is your sterilization procedure? I would love pics of your tc area
    Great work! I'd be interested in seeing your work area also. What do you do to control airflow & mitigate contamination?
    All the best,
    Ron
    You must do the thing you think you cannot do. --- Eleanor Roosevelt

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  6. #30
    cpsammich's Avatar
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    That's amazing
    What happened with the ceph explant?

  7. #31
    sarracenia lover dionae's Avatar
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    What is your sterilization procedure? I would love pics of your tc area
    This time around I just used MS with PPM to sterilize everything. Now, I got a few contams but so far its working out okay. I do not have a LFH and I do all my TCing in my living room.

    i do hope that you're able to induce a callous with the heliamphora
    The heli seeds got contaminated. I even tried taking them out and resterilizing them without success. Its part of the reason I stopped using bleach and peroxide to sterilize explants.

    What do you do to control airflow & mitigate contamination?
    Make sure theres no fans on and the air is still as possible, lysol and PPM.

    What happened with the ceph explant?
    It died. Didnt get any contams just never grew and finally turned black. Waiting on my cephs to start putting out winter leaves then i'll be trying again.

    Thanks everyone for stopping by and commenting!

  8. #32
    Eats genetically engineered tomatoes Sig's Avatar
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    Still very impressive! In the living room really counts as "Home tissue culture". Nice work! Have you ever gotten nep seeds to grow out?
    Formerly known as Silenceisgod!

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