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Thread: Small tissue culturing success

  1. #33
    sarracenia lover dionae's Avatar
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    Thanks Sig! Nope, never had any nep seeds germinate. This is only my second try though. I've mostly just concentrated on VFTs.

  2. #34
    sarracenia lover dionae's Avatar
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    Smile Small Update

    Had a few sarr seeds germinate, along with lots of contamination. Sarracenia seed seem very difficult to sterilize. The flytraps that I replated are really growing fast...they were needing replating bad. Nothing on the nep seeds so far but no contams so my fingers are crossed.

    Dente explant



    Typical VFT explants, a green and a red





    S flava var rubricorpora x S flava var ornata in agar





    Same cross in gelzan




  3. #35
    Confused Magikarp fdfederation's Avatar
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    Very nice.

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    BigBella's Avatar
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    Nice effort all around!
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  5. #37
    sarracenia lover dionae's Avatar
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    Thanks fdf and BB!!!

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    Never Knows Best gill_za's Avatar
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    dionae,

    I am reading up on the TC in an effort to learn as much as I can to try it in the near future. I was curious if you could clarify a couple of things a bit:
    So you have used Murashige and Skoog media with PPM in it.
    What was your sterilization procedure (lysol and PPM)? Could you please describe it a bit?

    Thank you very much in advance!

  7. #39
    sss's Avatar
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    Wow nice pictures. Do sarr seeds grow faster when grown this way?

  8. #40
    sarracenia lover dionae's Avatar
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    What was your sterilization procedure (lysol and PPM)?
    The lysol is for cleaning surfaces etc. I use 1/3 MS and a PPM solution to sterilize the explants. Last run I used 2ml PPM in 4 oz of 1/3 MS. Not too many contams...actually none on the heli seed and ceph explants which were the last things I plated. Even have a callus forming on a ceph leaf finally. 1ml BAP per liter of media is what I used to initiate the callus.

    Do sarr seeds grow faster when grown this way?
    Not for me but they divide like crazy.

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