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Thread: my first 2 batches of sterile cultures, neglected, but still alive

  1. #1
    CPlantaholic's Avatar
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    Red face my first 2 batches of sterile cultures, neglected, but still alive

    Hey all,
    After over a year of delay, I finally managed to get some sterile cultures going last March and in early May. It sure is convenient to have access to a lab with flow hoods and ethanol/supplies! The jars and gel/nutrients are all purchased by me though.

    I was in san diego all summer so i made these cultures a week before i left, placed them under my lights with the rest of the plants in the basement (~80-85F in summer), and then I came back after 3 months to see what happened .

    I decided to use a ton of media in each of the jars so that they could hopefully live longer after germinating.
    I should've used more gelrite since the gel was almost liquid after it solidified...so after 3 months, the plants used up all the nutrients and the gel became entirely liquid for many, even though I stirred the gelrite/nutrient melted mix really well before pouring most of the jars. Tips would be appreciated.

    but here's what i did if you're interested (I used 1.5-mL microcentrifuge tubes and vortexed them on the vortex genie):
    70% etoh rinse for all seeds, 2mins
    Larger seed size group- do 6 min vigorous vortexing in 10% bleach (ie D. capensis, D. indica)
    Med seed size group- do 6 min vigorous vortex in 5% bleach
    Small seed size group- do 4 min vigorous vortex in 1% bleach (ie for D. burmannii or sessifolia)

    My mix:
    1/3 MS with vitamins (full conc. Is 4.4g/L)
    30g/L sucrose
    2.0g/L Gelrite

    After spreading the seeds out with a pipetteman, I sealed the jars with micropore surgical tape, commonly used at ISU in tc labs.

    About 1/4 didn't germinate and 1/6 had contamination. hopefully i will get better with time. I will also be taking a graduate level horticulture class to learn about sterile protoplast culture, pollen culture (i think) and other tc techniques, which should be amazing!!!

    I am extremely appreciative of everyone's help- especially Dudo, Matt, and anyone who helped answer my annoying questions

    So enough talk...here are the pics:




    D. anglica


    D. anglica var. 2


    D. ascendens


    not all the plants made it...this jar didn't have enough media in it to keep them alive


    D. brevifolia


    D. collinsiae


    D. indica from my 1st attempt:


    I should've stored it in the fridge, cuz it ran out of nutrients


    D. nidiformis


    D. sessifolia gone wild!


    D. tokaeinsis jar 1 (just a little bit overcrowded )


    D. tokaeinsis jar 2


    D. tomentosa


    VFT (the only seed that germinated)


    typical D. capensis, grown in my 1st attempt and stored in the fridge for 3 months


    All sundews will be used for/by the ISU CP Club that my friend (forum name peat) and I started.

    Thanks for looking!
    Aaron
    Visit The Sundew Grow Guides: http://www.growsundews.com
    New- Drosera video tours & other sundew info, now on YouTube!

    Happy Growing!

  2. #2
    i dont do pots. amphirion's Avatar
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    dang it....i wish my school had a CP club....i guess we're not as cool as you. your CP club is getting spoiled SILLY! super jealous!
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  3. #3
    mass's Avatar
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    D. ascendens!!!
    Good god man, share that wealth.. ~puppy dog eyes~

  4. #4
    Class 5 Nepenthes hoarder lance's Avatar
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    Wow those are some cool sundews!!

    Good god man, share that wealth.. ~puppy dog eyes~
    I agree


    In addition to growing plants, I design and build RC planes powered by Tesla batteries. Check out my progress at www.chargedplanes.com

  5. #5
    BigBella's Avatar
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    Great effort . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Not Growing Up! GrowinOld's Avatar
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    Aaron,
    Good to see you are still going at it!
    Plants are looking good! As always!


    "Yo" to you & Phil! It's been a long time!


    Paul
    GrowinOld


    Personally, anyone who knows you, realizes you have already shared the wealth!
    Experience is the best teacher. At least it used to be.
    But then, common sense isn't so common anymore, is it.


    http://www.terraforums.com/forums/sh...d.php?t=113866

  7. #7
    instigator thez_yo's Avatar
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    Oh nice, sundew-master

  8. #8
    I hate bugs. Carnivorous plants get me. jpappy789's Avatar
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    simply stunning! Cruising around your website the rest of the collection looks amazing as well
    -Josh
    Grow list

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