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Thread: Few TC Questions

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    sarracenia lover dionae's Avatar
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    Question Few TC Questions

    I was curious about mixing 2 rooting hormones together in the media. For example, can i mix IBA and NAA?

    Activated charcoal-why/when is it used? Does the origin of the charcoal matter(wood, coconut husks, etc.)?

    Has anyone used any other auxins or cytokinins than the regular kinetin, BAP, NAA, IBA?

    Thanks!

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    BigBella's Avatar
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    Some custom medias contain a variety of both auxins and cytokinins; and the addition of coconut water muddies that issue even further, since it already contains a variety of plant growth regulators (in addition to sugars, sugar alcohols, amino acids, purines, vitamins, etc). Also, both auxins and cytokinins, depending upon their concentration, type and plant species involved, can either serve to promote or inhibit, to some degree, one another.

    Activated charcoal (use only a "pharmaceutical" or TC-grade) is solely from wood and is very absorptive of toxic substances in media -- such as phenols exuded from explants; and other compounds, for example, 5- hydroxymethyl-furfural (HMF), a product derived from sucrose through autoclaving media under acidic conditions; and to remove fatty acids from bacteriological cultures. The drawback is that it also removes promotory substances, especially auxins; charcoal also contains a small amount of PGRs; and it can raise the pH of media by .75-1.0. It is also used to minimize or exclude light from cultures to promote various dark-related functions, such as rhizogenesis (root formation), etc.

    I have also used all manner of plant growth regulators and additives, including auxins, cytokinins, ethylene, gibberellins, phenols, polyamines, steroids; organic (citric, lactate, malic, et al) and abscisic acids, to name a few . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    sarracenia lover dionae's Avatar
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    Thank you very much david! That really helps.

    Another question though. Why would I only use pharm/TC grade charcoal? Seems its made from the exact same thing as edible AC. Thanks!

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    BigBella's Avatar
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    Quote Originally Posted by dionae View Post
    Thank you very much david! That really helps.

    Another question though. Why would I only use pharm/TC grade charcoal? Seems its made from the exact same thing as edible AC. Thanks!
    Activated charcoal is prepared through the controlled carbonization of wood in either steam or air; and some lesser charcoal products may yet contain inhibitory compounds and byproducts. Those produced for the scientific market are a far more consistent grade than crap from some health food store . . .
    Last edited by BigBella; 12-27-2012 at 09:02 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    sarracenia lover dionae's Avatar
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    Thanks again!

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