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Thread: I'm taking a Tissue Culture course next semester, any advice?

  1. #9
    divaskid's Avatar
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    BigBella, great inspiration! Its getting me excited for school to start again. How long did it take to get your ceph that size?

    Edit: is it true no one has done tissue culture of Nepenthes through explants, only by seeds?
    Last edited by divaskid; 12-28-2012 at 12:29 AM.
    ~Michelle (AKA Geva or Jennifer)

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    Quote Originally Posted by divaskid View Post
    How long did it take to get your ceph that size?
    Edit: is it true no one has done tissue culture of Nepenthes through explants, only by seeds?
    In answer to your first question, that Cephalotus was established in Spring 2011; though the seed in culture had been previously cold-stratified for eight weeks . . .

    In answer to the second: no, it can done; but the process often involves twenty-fours hours or more of soaking the explant in broad-spectrum, many now-illegal algal and fungicides -- and the partial dissection of the Nepenthes to allow the chemicals to reach the deeper-affected tissue (so as to kill the endosymbiotic algae). I have done it on a few occasions; but I was just as likely to kill the explant as to establish it; more so in fact.

    In the short term, I would concern myself with seed; there is plenty to learn from the vagaries of in vitro germination . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    BigBella's Avatar
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    Quote Originally Posted by pokie22 View Post
    What concentration of PPM and for how long? 8% for 3-4 hours?
    Eight percent for six hours at the very least, up to ten; and expect a good deal of phenolic bleeding afterwards. I generally replate twice before it ceases . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Will work for CP's Kinjie's Avatar
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    Quote Originally Posted by BigBella View Post
    In answer to your first question, that Cephalotus was established in Spring 2011; though the seed in culture had been previously cold-stratified for eight weeks . . .

    In answer to the second: no, it can done; but the process often involves twenty-fours hours or more of soaking the explant in broad-spectrum, many now-illegal algal and fungicides -- and the partial dissection of the Nepenthes to allow the chemicals to reach the deeper-affected tissue (so as to kill the endosymbiotic algae). I have done it on a few occasions; but I was just as likely to kill the explant as to establish it; more so in fact.

    In the short term, I would concern myself with seed; there is plenty to learn from the vagaries of in vitro germination . . .
    David, I was wondering what times your classes started in spring cause I would really like to sign up and learn this stuff from a master of TC. All your stuff always looks great!

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    Quote Originally Posted by Kinjie View Post
    David, I was wondering what times your classes started in spring cause I would really like to sign up and learn this stuff from a master of TC. All your stuff always looks great!
    new business, er front, for the real business

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    BigBella's Avatar
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    Quote Originally Posted by Kinjie View Post
    David, I was wondering what times your classes started in spring cause I would really like to sign up and learn this stuff from a master of TC. All your stuff always looks great!
    You're far too kind. I began in TC because I was the "new kid" at the time and low on the totem; the giant lab manuals were foisted upon me (the damn things looked like the Manhattan white pages); and I then had more patience and attention span than the guys regularly treating their "glaucoma" behind the lab . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    divaskid's Avatar
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    Quote Originally Posted by Kinjie View Post
    David, I was wondering what times your classes started in spring cause I would really like to sign up and learn this stuff from a master of TC. All your stuff always looks great!
    I second that!
    ~Michelle (AKA Geva or Jennifer)

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