User Tag List

Informational! Informational!:  0
Likes Likes:  0
Page 1 of 2 12 LastLast
Results 1 to 8 of 15

Thread: I'm taking a Tissue Culture course next semester, any advice?

  1. #1
    divaskid's Avatar
    Join Date
    Jun 2004
    Location
    Hawaii
    Posts
    723
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)

    I'm taking a Tissue Culture course next semester, any advice?

    Hi,

    I'm going to start a tissue culture course next semseter (lasts a year or longer) and I was wondering if anyone had any advice before I start. I'd like to be as successful as possible so the more information the better

    I'll be doing TC on a nepenthes (unkown type so far) and also cephalotus. Both I've read aren't the easiest (especially the cephalotus), but the teacher gave me the go ahead on them. Does anyone have any advice or experience with these that would help me down the right path?

    Thanks
    ~Michelle (AKA Geva or Jennifer)

    Grow/Trade List

    Picture Thread

  2. #2
    kulamauiman's Avatar
    Join Date
    Jul 2009
    Location
    Kula, Maui USA
    Posts
    1,921
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    start them as seeds. Neps are a pain apparently to get explants cleaned....

    assuming similar for the cephs....

  3. #3
    sarracenia lover dionae's Avatar
    Join Date
    Nov 2010
    Location
    KY
    Posts
    1,963
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Cephs are hard. I've only been successful once with several tries.

  4. #4
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,972
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Cephalotus are a breeze to culture, both from seed and explant. Use the PPM (Plant Preservative Media) process to clean the explant or seed and a dilute 1:3 MS or 2:3 Knudsen-C media with or without PGRs.

    Check Matt's TC forum at www.flytrapcare.com; it is a valuable resource . . .

    Cephalotus follicularis "Eden Black x Self" (seed)
    Last edited by BigBella; 12-27-2012 at 08:57 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  5. #5
    sarracenia lover dionae's Avatar
    Join Date
    Nov 2010
    Location
    KY
    Posts
    1,963
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    What part of the ceph do you use? I'm 1 for 10 with leaves.

  6. #6
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,972
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Quote Originally Posted by dionae View Post
    What part of the ceph do you use? I'm 1 for 10 with leaves.
    Leaves with the rhizomal -- white -- tissue remaining on the leaves and PPM for sterilization. The most common mistake involves killing the explant with alcohols and bleach . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  7. #7
    sarracenia lover dionae's Avatar
    Join Date
    Nov 2010
    Location
    KY
    Posts
    1,963
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Thats a beautiful culture. Thanks for the tips!

  8. #8
    pokie22's Avatar
    Join Date
    Sep 2012
    Location
    Bay Area, CA
    Posts
    324
    Mentioned
    0 Post(s)
    Tagged
    1 Thread(s)
    What concentration of PPM and for how long? 8% for 3-4 hours?

Page 1 of 2 12 LastLast

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •