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Thread: Cheap Tissue Culture

  1. #73
    Sundrew's Avatar
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    Thanks for all the advice. What else do you add to your media besides fertilizer and agar? At the moment I have 10 jars that were given to me for free, but I will certainly check out the dollar store in the future. Thanks for your advice!

  2. #74
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    Basic: light + temperature + agar + sugar + fertilizer
    Google for “The Components of Plant Tissue Culture Media I Macro & Micro” for more information about macro and micro nutrient.

    Enhance: vitamin, inositol, potato/banana/tomato extracts, amino acids, organic acids …
    Google for “The Components of Plant Tissue Culture Media II organic addition”; review it, compare with MS media, and add supplements if you can to improve your medium.

  3. #75
    Sundrew's Avatar
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    @Flasker: You can see the medium I use in the previous page of this thread. Thanks for the suggestions, I'll read those papers for my next attempt.

    WOOHOOO!!! I completed the first five jars of media today. I took out half a cup of the liquid and added 6 teaspoons of cornstarch. I used a syringe to transport about 30mL to every jar. I boiled them in the pressure cooker for about 30 minutes, and it all went without a hitch. One jar opened when I was trying to pull it out, which is unfortunate, but I closed it as quickly as possible. All of the jars looks great, there was some stray liquid in one or two jars, but since cooling they have solidified completely. They are currently Saran-wrapped in my basement awaiting cuttings (probably planted by Tuesday). I'm so excited. On my blog I will have a video quickly showing the process up by Tuesday.


    Pics:

    The whole batch.

    DSC02225


    With flash.

    DSC02227


    The real color without flash. Not that gross!

    DSC02228

  4. #76
    Lucky Greenhorn Lil Stinkpot's Avatar
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    You gonna start selling these? Or should we just send you the cuttings/seeds?
    If you shake a rain stick, you get rain. I need a hamata stick.
    My WWWs

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    The jars remind me of an odd dessert I had in a Asian restaurant -- sort of a pudding made with agar with almond flavoring.

    How does the media taste?
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #78
    Sundrew's Avatar
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    LOL if I had the experience to tell you I'd be knocked out on the floor from the ferts...

    Someone else told me it looked just like where Miller and Skoog first found cytokinins.

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  8. #80
    BigBella's Avatar
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    Quote Originally Posted by Sundrew View Post
    LOL

    Someone else told me it looked just like where Miller and Skoog first found cytokinins.
    Jello® Spoiled Herring Semen Parfait?
    Last edited by BigBella; 03-17-2013 at 09:57 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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