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Thread: Cheap Tissue Culture

  1. #25
    Sundrew's Avatar
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    Liquid nutrients done! The pressure cooker will be bought later today, as well as some corn starch to play around with as a gelling agent.

    The materials:

    DSC02169


    The result:

    DSC02165


    It's currently in a pitcher in my freezer, until I use it next week.

  2. #26
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    Drew,

    Just remember that the media will all look like water when it is first removed from the pressure cooker; and that it will only gel once it completely cools. That may take a bit longer -- the gelling -- for the corn starch . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  3. #27
    Sundrew's Avatar
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    Thanks for the advice. Do you think I should wait a full day before opening and adding explants? Also, would you recommend adding the agar/corn starch and then dispensing to the jars, or dispensing to the jars first and then adding agar/corn starch individually? I read on FTC that the latter is a more reliable method.

  4. #28
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    Drew, mind sharing the recipe/proportions you used for the nutrient?

  5. #29
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    Everything can be found here: http://sundrew.blogspot.com/


    But this was the recipe, as directed to me by BigBella:

    Table sugar (sucrose): 1/8 cup
    Distilled water 1 cup
    10:10:10 fertilizer (1/4 teaspoon dissolved in one gallon of water). Use 1/2 cup of that mix
    Inositol tablet (250 mg) -- use 1/2 of tablet
    Vitamin tablet with thiamine -- use 1/4 tablet
    Agar flakes (amount varies with manufacturer)

    I diluted it by 1/2 because I'm working with CPs.

  6. #30
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    Drew,

    Mix everything together, since the quality of corn starch varies and it is better to have it completely dissolved, then having globs of it suspended in media. Also, autoclave it at the higher end, in terms of time. Gelling agents other than gelzan and agars can be "temperamental" at times, in terms of completely dissolving. I would do a small trial amount and see what happens.

    Also, be damn well careful when futzing with pressure cookers . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  7. #31
    Sundrew's Avatar
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    Ok, I'll be sure to do a few trials. It'll be really cool if it works! In terms of pressure cookers, I got a little nervous when this guy behind me in line at Walmart said that he "Hated the aluminum pressure cookers because they leak." Not the kind of comment I was looking for.

  8. #32
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    Quote Originally Posted by Sundrew View Post
    Ok, I'll be sure to do a few trials. It'll be really cool if it works! In terms of pressure cookers, I got a little nervous when this guy behind me in line at Walmart said that he "Hated the aluminum pressure cookers because they leak." Not the kind of comment I was looking for.
    The cookers don't leak if properly maintained. Just make sure that the o-ring is intact, and, above all, clean. Just don't go into another room on a "Mackintosh workout" and not pay attention to it. Read the manual too . . .l
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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