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Thread: Cheap Tissue Culture

  1. #65
    Sundrew's Avatar
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    Anything for science I guess. I did do a search for kinetin and zeatin supplements, and found this http://www.amazon.com/Kinerase-Thera...eywords=zeatin. Hard to administer, though, and costs twice as much as this whole project combined.

    EDIT: I read a few papers, and it appears that seaweed extract also contains many cytokinins. One abstract of a study concerning commercial brands said that "Cytokinin activity of the seaweed extract was confirmed by the increase in growth it produced with tissue-cultured cells...The results obtained show that the seaweed extract has a cytokinin activity capable of producing physiological changes, even when applied at the low concentrations used in practice" (Brain). Maybe something like this could work? http://www.amazon.com/Maxicrop-Liqui...eaweed+extract Or maybe this? http://www.homedepot.com/p/t/2036159...N#.UUSuQ1vwIeA
    Last edited by Sundrew; 03-16-2013 at 10:40 AM.

  2. #66
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    I limited the earlier response to the question of supplements; but the use of all manner of foodstuffs have been attempted and shown to have some hormonal activity or influence growth, including orange juice, for example. It can promote callus growth in Citrus species, though it is thought to be the influences of organic acids more than anything else; and banana homogenates have been used for decades in orchid culture; though, whether it is the presence of PGRs in some of the substances; an aid to the Kreb Cycle, associated with organic acids; or, simply the stabilization of in vitro pH, thought to be associated with banana; the jury is still out . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  3. #67
    Sundrew's Avatar
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    That's really cool. So maybe I'll add a smoothie to the mix?

  4. #68
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    I just finished my first test with the pressure cooker. I pressurized three different baby food jars with different amounts of cornstarch for 15 minutes to see how it gelled. It went remarkably well (until I dropped one of the jars ). The jar with 6 teaspoons of cornstarch per 1/2 a cup of water gelled perfectly, so I think that is what I will use with the real deal tomorrow. I also bought some neon food coloring, so now it's a real mad scientist experiment!


    6 teaspoons per 1/2 cup of water:

    DSC02214


    5 teaspoons per 1/2 cup of water:

    DSC02213


    4 teaspoons per 1/2 cup of water:

    DSC02212


    Having a pressure cooker on high heat for too long is one of the scarier experiences in life...

    from Drew E on Vimeo.


  5. #69
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    I'd advise avoiding any food coloring in the media, until you get a handle on contamination -- which is a far greater concern. Colorings are generally added to code for specific media and aren't necessary for the time being.

    Oh, and stop dropping things . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #70
    Sundrew's Avatar
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    I thought that food coloring could help pick out contamination? Or does it spread as the same color?

    I'll try. Baby food jar lids have very few threads. 10 left to experiment with.

  7. #71
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    Sorry for another double post, but it is unrelated to the above. In reading about cytokinins, it appears that they are produced by the root tips of a plant in excess; roots supply all of these hormones for the entire plant. Do people use root tips in culture? Are they very difficult to sterilize?

  8. #72
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    Some suggestions:
    • Fertilizer: choose fertilizer with completed macro and micronutrient (N,P,K,Ca,Mg,Mo,Zn,Mn,Cu,Fe) Try to avoid fertilizer with urea because heat may convert urea to harm chemical.
    • Buy agar pouch at Asian market for $1.50.


    • Use pickle/jam/pepper … jars from dollar store, 1 jar 1 dollar. They look good, cheap and much better than baby food jar.



    • You need a digital 100g x 0.01g balance from Ebay for less than $10.
    • Now, it's time for practice, try seeds first: sterilize and sow seeds.

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