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Thread: Kitchen culture kits

  1. #9

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    I am working on getting all the basic equipment needed. I bought a pressure cooker just for that job. I am planning on using baby food jars and have collected over 60 jars so far. Not that I will be using all of them to start. The other basic supplies are either on hand or will be on the way when I order the KCK.

    I have read that Sarracenia seed is hard to sterilize. Has anyone tried to removing the embryo from the seed for introduction into tissue culture?



    Nick

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  2. #10

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    Excision of the embreyo! I do it with citrus and it speeds germination,also I`m trying it with apple\pear seeds.
    [img]http://home.**********.com/users/pondboy/Neps/Neps%20sig..JPG[/img]

  3. #11

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    Today, after about three weeks from my first attempt, I noticed a seedling! It's from a snapdragon seed... however, it's in one of my contaminated cultures, so it's a race against time. Exciting though!

  4. #12
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    A good, easy to get hold of book is Plants from Test Tubes; an Introduction to Micropropagation by Lydiane Kyte & John Kleyn. I have the hardcover 3rd edition which was about $40 but recently Timber Press has come out with a softcover edition for half that much! [img]http://www.**********.com/iB_html312/non-cgi/emoticons/sad.gif[/img] This book tells you just about everything you need to know about TC. You can use the plans in this book to make your own stuff or buy premade parts and setup an entire TC facility. A very useful and versitile book!

    Also, you might check out Phytotech Laboratories website for info and higher quality growing medias, antibiotics and hormones and even TC dry goods like flasks, tools and sterilizers than the "hobby lab" geared places.

    Good luck! [img]http://www.**********.com/iB_html312/non-cgi/emoticons/smile.gif[/img]

  5. #13

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    Advantage to tissue culture? How fast do you get a nice big clone? I know we clone carrots here at my school and it takes a whole semester to get a little baby carrot so I thought I would ask this.
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  6. #14

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    I bought "Plants from Test Tubes" about 4 months ago.

    Rick Walker has some great stuff on tissue culture of Drosera and Pinguicula. The suggestion that using hormones is not really needed in home tissue culture unless mass multiplication is needed. Has anyone used the coconut milk culture. I will post a link later as I am not able to access it from work.

    What I am interested in is Nepenthes, Sarracenia, and Dioneae as my main goals for culture.

    Thanks,

    Nick
    Nick

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    http://www.carnivorium.com
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  7. #15

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    I haven't tried it, but asked a Sarracenia "expert" once about using Sarracenia seed for TC. He said that it's actually quicker to raise Sarracenia from seed the normal way, provided you fertilize them biweekly and skip dormancy for the first year or two. In other words, you'll get a mature plant quicker. The only advantage to TC Sarracenia seed would be that you would be able to propagate them quicker once you get them going.

  8. #16
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    I have this book also.

    Darcie:

    TC really speeds things up if you are starting from seed, you can have 3 year old size sarracenia in a matter of months. However, the main advantage is the rapidity by which you can produce new clones. With the correct conditions, etc., one can have hundreds and thousands of plantlets from one leaf in a few months.

    -noah

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