Has anyone used the Kitchen Culture Kits for CP use? I am thinking about trying the kit and would like to know if anyone has had success with these tissue culture kits.
I haven't...yet, but I'm saving up money to buy a cp kit from there. [img]http://www.**********.com/iB_html/non-cgi/emoticons/smile.gif[/img] I'm absolutely sure it would work. the only way it wouldn't work is if you don't do it properly and don't sterilize the flasks good enough, then you get fungus... [img]http://www.**********.com/iB_html/non-cgi/emoticons/wow.gif[/img] [img]http://www.**********.com/iB_html/non-cgi/emoticons/sad.gif[/img]
Hello everyone, can someone tell me were you can buy this kit from I am rellay interstead in purchasing this kit can some one help me out. Thanks for replys. [img]http://www.**********.com/iB_html/non-cgi/emoticons/biggrin.gif[/img] [img]http://www.**********.com/iB_html/non-cgi/emoticons/smile.gif[/img]
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I am really thinking about getting the kit for cps. Has anyone tried the kit that is sold with Sarracenia? I have some Sarracenia alabamensis seed that I would like to try. Of course, I am going to try S. purpurea or another common species first just to see how it works.
I used the kit. It is great for starting tc if you are willing to put in the work and effort. They suggest microwave use, but be warned that this only sterilizes roughly half of the cultures - a pressure cooker or autoclave is almost mandatory if you want to do this successfully long term.
Sarracenia tc is difficult, as you have to introduce the plant using either rhyzome tissue or seed. Seed sterilization is difficult due to roughness of seed, and rhyzome meristem isolation is also very difficult and risky, especially when one is beginning.
I suggest that if you pursue tc you start with droseras or pinguiculas, which are easy to start from leaf tissue, or use seed for almost any genus.
if you'd like to know more feel free to contact me. [img]http://www.**********.com/iB_html312/non-cgi/emoticons/smile.gif[/img]
I actually pieced together tissue culture stuff from around the house. Still thinking of getting a pressurecooker, though, as it appears about half my cultures have mold.
I haven't tried with carnivorous plants yet though. Still waiting to see if it worked, or if I need to change something else. African violets are supposed to be very easy to tissue culture, so that's what I started with.
I am working on getting all the basic equipment needed. I bought a pressure cooker just for that job. I am planning on using baby food jars and have collected over 60 jars so far. Not that I will be using all of them to start. The other basic supplies are either on hand or will be on the way when I order the KCK.
I have read that Sarracenia seed is hard to sterilize. Has anyone tried to removing the embryo from the seed for introduction into tissue culture?
Excision of the embreyo! I do it with citrus and it speeds germination,also I`m trying it with apple\pear seeds.