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Thread: Tissue culture

  1. #9
    Nepenthes Specialist nepenthes gracilis's Avatar
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    Hey noah. All I need to know is how to do tiisue culture period. The main things I need to know are: The media and how to make it for all CP's. How to sterilaze stuff. How to do TC like with leaves,seeds,etc. Anything else is helpful and gladly accepted. Nep.G.

  2. #10
    Guest
    See...

    There are more people other than me that can help you.

    Although I do appreciate the kind words for me! =)

    I sterilize baby food jars in a pressure cooker. I know that it sounds weird...but I haven't the money for an autoclave or something like that. The pressure cooker works fine for me.

    As far as the medium...I get it pre-made from a company and I couldn't even begin to tell you what all is in it.

    But when you are doing this ( if you plan to )...you need a sterile room( or place). Or else...you will grow everything else that is in the air. That would be a lot of stuff!!!

  3. #11
    Nepenthes Specialist nepenthes gracilis's Avatar
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    I was going to say jaie. If You didn't appraciate those kind words then I would classify you as wierd. Everyone appreciates kindness [img]http://www.**********.com/iBhtml/non-cgi/emoticons/smile.gif[/img] Of course too much may kill somone! [img]http://www.**********.com/iBhtml/non-cgi/emoticons/smile.gif[/img] Killing with kindness. I think TC will be too expensive and too complicated so i will propagate stuff the old fashioned way. It is just too cmplicated and I know I couldn't do it because I just know I would get the media wornd I wound't strilize enough or get in bactria. Ohh TC isn't fair! Nep.G.

  4. #12
    NikHiner
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    I buy my media from: http://www.kitchenculturekit.com/catalog.htm

    I use them for Hosta media but they have a CP media. I think it is around 15.00.

    Hope this helps.


    (Edited by NikHiner at 8:32 am on Dec. 14, 2001)

  5. #13
    noah's Avatar
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    Yup, same here, 1/2 strength MS media works great for most cp's. And NG, I'm sure you could do it if you tried, come on, I'm doing it and I'm only 16. So there [img]http://www.**********.com/iBhtml/non-cgi/emoticons/smile.gif[/img] It can get a little expensive, but you can offset costs by selling or trading the plants you produce this way.... assuming you are successfull.

    I use a microwave to sterilize my baby food jars, it works fine. An emptly aquarium will do as a "clean area" and most of the other tools needed you probably already have in your house. If you (or anyone else) has any other questions regarding tc email me.

    Noah

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