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Thread: Micropropigation from seed help

  1. #1

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    Unhappy

    Ok, I got my pressure cooker, I know how to do micropropagation from plant leaves, no I need to find out how to do it with CP seeds.

    Do I starelize the seed and just throw it on a petri dish and watch it grow?

    Do anyone have advice, etc?

    OH!!! LOOK! NEW SMILIES! I've got to use them...let see.

    Clockwork Orange! [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_m_32.gif[/img]

    Ugh...American Idol.... [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_t_32.gif[/img]

    WASSS UPPPPP!!!!!! [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_n_32.gif[/img]

    ok, now that that's out of my system...
    LACPS Member
    \"Don't make me sic my targ on you!\" -Jonathan Passow
    www.mulletman.org <--VISIT MY NEW HOMEPAGE&#33;

  2. #2

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    Sterilising is very hard. You might want to stratify first. Here is the protocol I will be using:

    Rinse in sterile R/O water (you gotta pressure cook it), room temperature.
    Rinse in 10% bleach
    Rinse in sterile R/O water, room temperature
    Soak in 10% bleach for 5 minutes
    Soak in 70% alchohol for 2-5 minutes
    Rinse in 5% bleach, Some prefer not to wash the bleach residue off, as it helps keep dampoff away, etc

    There is a gradual PPM breakdown once you use the medium. Though the rate is
    variable and depends on the number of ex-plants per given volume of medium.
    Therefore there is a chance that after long period of culture infiltrated
    microbial spores will manage to germinate. However this is more likely to
    happen in solid medium.

  3. #3

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    What does R/O mean? [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/confused.gif[/img]
    LACPS Member
    \"Don&#39;t make me sic my targ on you&#33;\" -Jonathan Passow
    www.mulletman.org <--VISIT MY NEW HOMEPAGE&#33;

  4. #4
    Somewhat Unstable superimposedhope's Avatar
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    Reverse Osmosis, I think he means

    Joe
    \"There is nothing here of interest to any nation, as a matter of fact there is nothing here but humans&#33;\"

  5. #5

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    OH! ok, got it!
    LACPS Member
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