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Thread: Helimaphora from seed: old school and new school . . .

  1. #49
    i dont do pots. amphirion's Avatar
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    @lance: yes.
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
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    Class 5 Nepenthes hoarder lance's Avatar
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    How exactly is this done?


    In addition to growing plants, I design and build RC planes powered by Tesla batteries. Check out my progress at www.chargedplanes.com

  3. #51
    i dont do pots. amphirion's Avatar
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    @lance: same way how the heliamphora seed are...you have the TC media prepared, with cleaned/sterilized seed, and that is placed in the media.
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
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  4. #52
    BigBella's Avatar
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    Quote Originally Posted by lance View Post
    How exactly is this done?
    I would encourage you to check out the tissue culture forum at www.flytrapcare.com, where there are overviews of the process, which involves making the solid or liquid media; the adding of hormones; and the practice of sterile technique. It can be fairly involved . . .

    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  5. #53
    Plant Ninja Smitty's Avatar
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    good afternoon BigBella. I was wondering if you ever tried to germinate heli seeds in a ms media before? Ran out of knudson and need to do these cultures asap. Thank you sir for the marvelous updates.

    Previously known as: NY Plant Nerd

  6. #54
    BigBella's Avatar
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    Thanks . . .

    In the past, I have had some success with the seed and 20% MS media with vitamins, though the Knudsen-C has been far better. I can generally make a batch of the various media in the course of a couple of hours and keep quite a bit on hand . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  7. #55
    Plant Ninja Smitty's Avatar
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    Thanks for the report BigBella. I am sorry I didn't see the response sooner, pretty rude of me!

    I went ahead and ordered 10 liters of knudson c media to get me a nice little head start on making extra cultures ahead of time...Lesson learned!

    Previously known as: NY Plant Nerd

  8. #56
    BigBella's Avatar
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    Ten liters is a ton of material to have on hand and you'd be better served by ordering just what you require. Even the powdered media in its sealed foil envelopes has a finite shelf-life; and a single liter can fill dozens of test tubes -- and I seldom ever make more than a liter batch . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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