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Thread: "Eden Black" -- F1

  1. #65
    BigBella's Avatar
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    Quote Originally Posted by rjgeorgia View Post
    I was wondering how the EB - F1 seeds have fared? I am entering this thread a bit late, but was also curious what media you used, some variation of ms? Have you tried ceph meristem? Any luck avoiding phenolic oxidation?
    Here is a photo of a root-forming callus (as opposed to those producing shoots or leaves) of the "selfed" seed mentioned earlier. Root formation is often the weakest link in terms of tissue cultured plants; and commercial growers -- to cut costs -- often transfer "plantlets" of many species to compost to root ex vitro. I have found that Cephalotus, in particular, is far more successful and vigorous if there is something more substantial below the soil . . .

    Cephalotus follicularis "Eden Black x Self"
    25 August




    I was also very encouraged by the bright red coloration of the earliest "vegetative" leaves on another callus, even under the moderate lighting conditions of a growing room:

    Last edited by BigBella; 08-27-2012 at 02:05 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Decumbent Fanatic Jcal's Avatar
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    That's for the update. I keep checking this thread to see how everyones seedlings are coming along. I'm excited and I don't even have one!

  3. #67
    BigBella's Avatar
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    Here are a couple of other shots of root-producing calli in Muroshige-Skoog-based multiplication media -- both of which currently look like something out of a Lovecraft story:

    Cephalotus follicularis "Eden Black x Self" 28 August



    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    mass's Avatar
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    it's alive... ALIVE!!

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    Thats Freakin awesome David. Way far ahead from where my seedlings are.

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    BigBella's Avatar
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    Quote Originally Posted by Rob View Post
    Thats Freakin awesome David. Way far ahead from where my seedlings are.
    Thanks Rob. Here's a shot of one of the "explants" producing something which actually resembles a plant:

    Cephalotus follicularis "Eden Black x Self" 30 August
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    BigBella's Avatar
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    Here is an update on some of the cultures. While somewhat difficult to see in the photos, I have been encouraged by the depth of color in the first flat "vegetative" leaves (seen as a fringe along the top of the 18 September photo) -- produced after transfer to a media without any plant growth regulators (PGRs). They are of a deep, deep red, similar to that seen in a kidney bean . . .

    Cephalotus follicularis "Eden Black x Self" 28 August




    18 September



    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  8. #72
    hcarlton's Avatar
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    Looks like some wierd alien spider growing in there! Great color forming already though!
    Everything has a reason, whether big or small. Never underestimate the power of what is or is not.
    There is far more to everything than meets the eye.
    Growlist

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