Yeah, I saw that; but since I received seed a little late in the game; was concerned for its viability, and pressed for time -- decided upon the TC route . . .
TerraForums Venus Flytrap, Nepenthes, Drosera and more talk
Register a free account today to become a member! Once signed in, you'll be able to participate on this site by adding your own topics and posts, as well as connect with other members through your own private inbox!
"Eden Black" -- F1
- Thread starter BigBella
- Start date
Yeah, I saw that; but since I received seed a little late in the game; was concerned for its viability, and pressed for time -- decided upon the TC route . . .
Please don't get me wrong, I wasn't questioning your motive for TC... just found it really interesting on the speed these seeds have matured in traditional cultivation.
Hi BigBella, I am new to terraforums and am a tcer myself. I focus on asymbiotic germination of Cypripedium species (lady slippers), however I have been dipping my hands into the tissue of cps as well. I was wondering how the EB - F1 seeds have fared? I am entering this thread a bit late, but was also curious what media you used, some variation of ms? Have you tried ceph meristem? Any luck avoiding phenolic oxidation?
The Eden Black x Self seeds have done well and several have formed friable root or shoot-forming calli (I'll post photos when I have the chance); and the media have ranged from dilute MS (1:4 or 1:3) with NAA; to 2:3 Knudsen-C; to a proprietary, low ammonium media being developed. While I haven't used meristematic tissue for TC, I have successfully used "vegetative" leaves of Cephalotus; juvenile pitchers; flower stalks; seed and rhizome tissue.
Phenolic "issues" can largely be avoided by handling the explants carefully during sterilization and by frequent re-plating (often within the same container) should discoloration occur; it eventually stops, though many tropical and sub-tropical plants are particularly high in phenolic compounds . . .
Phenolic "issues" can largely be avoided by handling the explants carefully during sterilization and by frequent re-plating (often within the same container) should discoloration occur; it eventually stops, though many tropical and sub-tropical plants are particularly high in phenolic compounds . . .
Last edited:
Here is a photo of a root-forming callus (as opposed to those producing shoots or leaves) of the "selfed" seed mentioned earlier. Root formation is often the weakest link in terms of tissue cultured plants; and commercial growers -- to cut costs -- often transfer "plantlets" of many species to compost to root ex vitro. I have found that Cephalotus, in particular, is far more successful and vigorous if there is something more substantial below the soil . . .
Cephalotus follicularis "Eden Black x Self" 25 August
I was also very encouraged by the bright red coloration of the earliest "vegetative" leaves on another callus, even under the moderate lighting conditions of a growing room:
Last edited:
Jcal
Decumbent Fanatic
Here is an update on some of the cultures. While somewhat difficult to see in the photos, I have been encouraged by the depth of color in the first flat "vegetative" leaves (seen as a fringe along the top of the 18 September photo) -- produced after transfer to a media without any plant growth regulators (PGRs). They are of a deep, deep red, similar to that seen in a kidney bean . . .
Cephalotus follicularis "Eden Black x Self" 28 August
18 September
Cephalotus follicularis "Eden Black x Self" 28 August
18 September
Lil Stinkpot
Lucky Greenhorn
lil hokie
heatherfeather knows the weather!
I was just thinking that it looked like an alien spider haha!!
GrowinOld
Not Growing Up!
Wire Man
Sphagnum Guru
You're so right! That totally looks like a baby tarantula!
