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Thread: Heli pollen observations

  1. #17
    Av8tor1's Avatar
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    Well, instant success would have been nice :P
    But I do love me some good French Onion soup on a cold winter's night

    I had some tatei pollen that i had froze using standard protocol with desiccant... got it out and made a slide of it with Leek epidermis

    so, while not uber fresh... it was stored in accordance with accepted techniques,

    surely one of these is going to show growth... umm maybe

    LOL

  2. #18
    Hello, I must be going... Not a Number's Avatar
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    You may as well do a graduated sugar concentration set while you are at it: 5, 10, 15 and 20% sucrose. Just label your slides
    Grand Hotel... always the same. People come, people go. Nothing ever happens.

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    Av8tor1's Avatar
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    yep.... i know it, and start looking for some cheap scales...

    already found cheap source for small quantities of boric acid (I probably already have it, use to use it as a flux) and calcium nitrate

    meh, if it was that simple it would have already been done i guess

  4. #20
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    Well if your 20% is pretty close you can take equal parts of the 20% solution and water to make 10%. And again with the 10% to make 5%. Equal parts of the 10 and 20% will give you 15%.
    Grand Hotel... always the same. People come, people go. Nothing ever happens.

  5. #21
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    Quote Originally Posted by Av8tor1 View Post
    yep.... i know it, and start looking for some cheap scales...

    already found cheap source for small quantities of boric acid (I probably already have it, use to use it as a flux) and calcium nitrate

    meh, if it was that simple it would have already been done i guess
    Laugh while you can, monkey boy, but I have found decent scales at ridiculously-cheap, Chinese prison labor prices on "schmee-bay."

    http://www.ebay.com/sch/i.html?_from...All-Categories
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #22
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    woohooooooooooooooooooooo!!!!!

    Ive got pollen tubes!!!!!!!

    so far, the onion epidermis is working... I wish i had the imager, man.... is it ever obvious. They are already many, many times longer then the pollen grain itself.

    Amazing that much growth can happen is such a short period.

    Fresh H. folliculata pollen with Spanish Onion epidermis = works!

  7. #23
    BigBella's Avatar
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    Congratulations -- soon the world will be yours. Please sign me up for a tour of your volcano lair . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  8. #24
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    Back to the fifth dimension buckaroo banzai

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