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Thread: Heli pollen observations

  1. #25
    RL7836's Avatar
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    Quote Originally Posted by Av8tor1 View Post
    woohooooooooooooooooooooo!!!!!

    Ive got pollen tubes!!!!!!!


    Very cool! Sounds like a candidate for time lapse....
    All the best,
    Ron
    You must do the thing you think you cannot do. --- Eleanor Roosevelt

    *** Growlist / Wants / Offers ***
    (with Pics)

  2. #26
    Av8tor1's Avatar
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    oh, i wonder if the software that comes with the imager is capable of doing it

    that would be so cool.....

    david, does your celestron software do time lapse?

    hmmm may have to do it manually so the light doesnt stay on and bake em...

    ---------- Post added at 06:14 PM ---------- Previous post was at 05:51 PM ----------

    Same Fresh H. folliculata pollen with 20% Sucrose (by volume) = no growth

    Early indications are the previously frozen H. tatei on leek is going to be positive for growth as well.

    It was started a couple hours behind the other samples but I think I see early signs of growth.
    (this would prove the freezing technique )

    Will verify in a couple hours.


  3. #27
    Hello, I must be going... Not a Number's Avatar
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    I would be surprised if it the software didn't. Even my toy Intel/Mattel/DigitalBlue USB microscope takes movies and timelapse shots. If Windows sees the imager as a TWAIN device you can find software out there to do it.

    Time to upgrade to a cold light source illuminator. I replaced the bulbs in my toy with ultrabright white LEDs but the driver was color corrected for the incandescent bulbs and there is no way to tweak color correction in the driver so everything is way too blue. Sometimes too way off to correct in Photoshop. Then if there is a mirror for under the stage you could always use that.
    Last edited by Not a Number; 11-20-2011 at 04:46 PM.
    Grand Hotel... always the same. People come, people go. Nothing ever happens.

  4. #28
    Av8tor1's Avatar
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    Leek epidermis + previously frozen H. tatei = no tube growth

    Not only is there no growth, but the pollen grains now appear deformed.
    They are no longer round but barrel shaped. I cant say for sure they weren't that shape when I made the slide. However, I think I would have noticed it, it is quite pronounced. I'm almost positive they were round when I checked on them a couple hours ago.

    It may have been a technique error as well, In my haste I didnt take time to thaw properly.

    Need to try frozen pollen with the onion epidermis and fresh pollen with the leek

  5. #29
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    Quote Originally Posted by Av8tor1 View Post
    Need to try frozen pollen with the onion epidermis and fresh pollen with the leek
    --Pink Floyd?
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #30
    Av8tor1's Avatar
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    Quote Originally Posted by BigBella View Post
    --Pink Floyd?
    By the way, which one is Pink?

    Oh, tracking shows imager should be delivered today, maybe be able to post some pics as early as tonight or tomorrow... Will have to start new sample first, they are very perishable me thinks. If I would've thought I would refrigerated the successful slide.

    I also got the scope cleaned, just needed a puff or two or 20 from squeeze ball air thingy


  7. #31
    instigator thez_yo's Avatar
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    So.. where's the pics of stuff in this thread? I'm the slow kid in the back of the class who's the visual learner...

  8. #32
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    Ladies and gentleman, let me introduce you to...
    (As far as I know, photographed for the first time)

    Heliamphora folliculata pollen tubes







    and a little movie showing the length of one of these tubes... sorry for getting my up/down/right/left confused a couple times






    thx for looking

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