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Thread: Heli pollen observations

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    Av8tor1's Avatar
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    Cool Heli pollen observations

    My slides and cover slips arrived today so I thought I would have a peek at the H. folliculata pollen I harvested on 11/6

    This pollen was dried overnight in a sealed container with ground up saltine crackers as a desiccant. The pollen was then froze for a few days, thawed and used to pollinate the next flower. Since that time it has sat at room temp in its sealed container. (Wouldn't surprise me if it wasn't still viable)

    My media was approx 20% sucrose by volume... (I don’t have any scales, I was hoping I wouldnt need to invest in any). I prepared a slide; applied the pollen, and made a growth chamber by placing wet paper towel in the bottom of a bowl with the slide suspended above. This was then covered in cellophane and allowed to sit at room temp for 6 hours.

    At 400x the pollen grains were easy to identify but no tubes were observed.

    Many of the references indicate the media may need some boric acid and calcium nitrate.

    I will try the plain sucrose media again with fresh pollen in an effort to isolate the problem. If they still don’t grow I will try fresh pollen using the onion membrane method.

    Still waiting on microscope imager to arrive... so no pics yet.

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    kulamauiman's Avatar
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    Hmmm. Much to ponder. I need to test the onion skin method at the office

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    Av8tor1's Avatar
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    I should be able to harvest some fresh pollen tomorrow.... that should tell me if the plain sucrose media has any hope of working

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    If only you could harness that scientific zeal for good and not evil . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Av8tor1's Avatar
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    Quote Originally Posted by BigBella View Post
    If only you could harness that scientific zeal for good and not evil . . .
    Give in to the dark side my friend.... we have cookies

    Well I thought I would look one more time this morning before cleaning the slide.... no change.

    On a related note, I love the microscope... much nicer than the basic Tasco I had as a kid.
    But all the optics are in need of a good cleaning

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    Hello, I must be going... Not a Number's Avatar
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    Why not try a control with pollen from a species that is known to work on onions skin?
    Grand Hotel... always the same. People come, people go. Nothing ever happens.

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    Av8tor1's Avatar
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    Warren,

    From what all I've read on the subject I'm not sure using another type of pollen would tell me much.

    It seems that what media works for one pollen type may or may not work for another.

    I got a Spanish onion and some leeks today; I will try a simultaneous run with onion, leek and 20% sucrose with uber fresh pollen.

    (From flower to slide)

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    kulamauiman's Avatar
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    20% seems like a lot of sugar. Seems it would cause cells to suffer from an osmotic imbalance .....

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