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Thread: Digestive fluid

  1. #9

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    There was a good paper printed in The Journal of Plant Physiology where they followed the expression of enzymes in Sarracenia purpurea. The title for the article is "Signal Transduction in the carnivorous plant, Sarracenia purpurea.

    As soon as the pitchers opened up, they filled them with sterile water containing several antibiotics in order to prevent bacterial contamination from giving a false enzyme reading. They enzymes they looked for were DNase, RNase, protease, phosphatase and chitinase. All were present except chitinase.

    Even though the newly opened pitchers contained only sterile water, they began to secrete enzymes. Without the addition of nutrients to the fluid, enzyme levels decreased. Old, inactive pitchers could respond to the presence of nutrients and begin to secrete enzymes again. In order to test this, they added either BSA protein, DNA, RNA, or various ionic solutes (such as reduced nitrogen), to the pitcher fluid. The article outlines the various methods that they used to test for enzyme activity.

    Brian

  2. #10

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    Noce! [img]http://www.**********.com/iB_html/non-cgi/emoticons/biggrin.gif[/img]
    thx for helping me out

  3. #11

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    correction; nice

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