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Thread: Tissue culture drosera

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    klasac's Avatar
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    Tissue culture drosera

    Hi! Recently I started pondering about learning TC for CP. I had some extra time on my hands in my lab and so I started trying out media and plants, most of them just died or got contaminated, but some time ago I got lucky and found a good medium for d. burmanii (seeds) and d. anglica leaves):




    Used modified home-made 1/3 MS medium, no phytohormones, no inositol. Not much success TCing other species, but still trying:-)

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    clippity-clip-clip Clue's Avatar
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    Cool. Somehow, tissue culture fascinates me.
    "I, for one, can't wait to grow Nepenthes extincta!"
    Plant List ; blog

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    Devon's Avatar
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    that's awsome, good luck and good job. I wish I could try TC some time but I have no clue how and I heard it's expensive... and I don't know how. :P

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    Tropical Fish Enthusiast jimscott's Avatar
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    Kewl!

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    klasac's Avatar
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    here are the same plants 3 weeks later:
    d.burmannii


    d.anglica generates roots (3 weeks later)


    Some explants, being on hormone free media, do not propagate, but continue regular growth (as if they have never been cut off from the plant). D. filiformis leaves continue unfurling and are dewy:


    and undeveloped flower stalk of d. rotundifolia developed, the stalk bloomed and now creates seeds!


    I guess some species need to have phytohormones present in the media.......any thoughts?

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    Learning How To Multiply Indigo's Avatar
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    I think my brain just drop dead....... its just amazed me that how much they have gotten in 3 weeks. and that leaf cutting is pulling out like 10 x few growth.... o.O

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    CPlantaholic's Avatar
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    nice work i don't know how you afford all the media/ supplies for your experimentation!!!
    Visit The Sundew Grow Guides: http://www.growsundews.com
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    Happy Growing!

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    klasac's Avatar
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    CPlantaholic: thanx:-) It is not expensive to buy MS media, hormones and bleach and agar. Although I do not buy MS media because I work in a chemistry lab and can prepare it, I checked the prices and the kits are not expensive at all :-)

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