User Tag List

Informational! Informational!:  0
Likes Likes:  0
Page 1 of 2 12 LastLast
Results 1 to 8 of 10

Thread: my latest TC developmoents (gelrite vs. agar)

  1. #1
    klasac's Avatar
    Join Date
    Jul 2008
    Location
    Myjava Slovakia
    Posts
    288
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)

    my latest TC developmoents (gelrite vs. agar)

    Hello!
    I would like to share some info and pics of my new TC jars. I have had issues with food store grade agar last fall and because several batches shifted the pH of the media several points up, I have lost some cultures. That was the last straw for me and I decided to upgrade my TC work to next level and thwart the drawbacks of using agar.
    Although agar (of high quality) can and does perform very well as a gelling agent, the main issue for me was that the gel was not clear enough to see the first sign of contamination early enough. It is vital for the survival of possibly contaminated culture that unaffected tissues are replated asap. Gelrite forms glassy crystal clear gel where even the smallest contamination can be spotted during initiation and the culture can be saved completely.
    Another good thing about gelrite is that unlike agar (even purest grade), it does not contain any organic compounds (such as phenolics) and therefore it does not stress replated tissues and they grow without interruption. It does contain some salts but i have not observed negative effects. And last thing is that you need only 1/5th of the amount of agar to form a firm gel:-)
    Here are some (agar and gelrite) based cultures:

    Drosera sp. 'lantau island'


    the same but on gelrite


    D. natalensis


    a very neglected propagation jar with D. peltata


    D. capensis (i use as a control), a shot through the sheer medium


    D. californica seedlings


    D. intermedia "Carolina Giant" seedlings


    D. neocaledonica propagation jar


    D. neocaledonica rooting jar


    D. ordensis propagation jar


    D. ordensis rooting jar (10 days past replating from jar in previous picture)



    D. falconeri


    D. hartmeyerorum


    D. afra
    Last edited by klasac; 12-18-2010 at 03:33 PM. Reason: Nomenclature adjustment

  2. #2
    Taliesin-DS's Avatar
    Join Date
    May 2009
    Location
    wezep, the netherlands
    Posts
    895
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    awesome!!
    my growlist: http://terraforums.com/forums/showth...306#post976306
    My pics: http://www.flickr.com/photos/taliesin-ds/

    <Exo> @Talie......You are the lord of all things blah....

  3. #3
    sarracenia lover dionae's Avatar
    Join Date
    Nov 2010
    Location
    KY
    Posts
    1,963
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    ^^^What he said!

  4. #4
    jonnyq's Avatar
    Join Date
    Sep 2010
    Location
    New York
    Posts
    225
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    This is breathtaking...

  5. #5
    Tropical Fish Enthusiast jimscott's Avatar
    Join Date
    Nov 2003
    Location
    Western New York
    Posts
    18,768
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    I wish I had your technology.... and chemicals!

  6. #6
    Moderator Joseph Clemens's Avatar
    Join Date
    Sep 2002
    Location
    Tucson, Arizona
    Posts
    2,539
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Very nice work, can you share more details of your protocols? Or are they proprietary?
    Joseph Clemens
    Tucson, Arizona, U S A

  7. #7
    klasac's Avatar
    Join Date
    Jul 2008
    Location
    Myjava Slovakia
    Posts
    288
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Hi all!

    Thank you for nice comments. For most of my TC I use 20-50%MS with some additives (depending on the particular species).

  8. #8
    Cosmo's Avatar
    Join Date
    Aug 2010
    Posts
    69
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Interesting.. and very clear indeed! In what amount per liter are you using the gelrite??

Page 1 of 2 12 LastLast

Similar Threads

  1. My latest additions
    By Shadow Chaos in forum General Discussions
    Replies: 10
    Last Post: 12-04-2008, 05:44 PM
  2. Envenomated agar? Any one try this?
    By swords in forum General Discussions
    Replies: 16
    Last Post: 03-07-2008, 10:11 PM
  3. Latest pics!
    By ludwig777 in forum Tropical Pitcher Plants  (Nepenthes)
    Replies: 5
    Last Post: 04-02-2006, 01:52 PM
  4. The wonder we call agar
    By superimposedhope in forum Tropical Pitcher Plants  (Nepenthes)
    Replies: 6
    Last Post: 11-08-2004, 01:39 PM
  5. Latest pitcher
    By chesara in forum Tropical Pitcher Plants  (Nepenthes)
    Replies: 12
    Last Post: 07-23-2004, 05:04 AM

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •