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Thread: D. regia

  1. #9

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    Zongyi_Yang: If you do your manipulation in a 'almost closed' container (such as a fish tank, on its side), and work close enough from a flame (ie alcohol lamp), you can achieve to reduce/avoid contamination to almost nothing. Of course, you must have a very clean area (ie the container) and washed your hands and arms properly before doing anything. Plus avoiding air circulation in your room during the transfert.

  2. #10

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    sounds difficult maybe one day i'll have enough to get a kit and try for myself
    Join the CCPS, you wont regret it: http://s4.invisionfree.com/CCPS

  3. #11

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    Zongyi_Yang

    To answer some of your questions:

    The jelly and air in the bottle are sterilized in a large pressure cooker. The work area ( table top, clean box and even the air in the room) are disinfected with a number of house hold chemicals such as rubbing alcohol & Lysol. Lastly the seeds are sterilized in diluted bleach and or hydrogen peroxide.

    So far I have had very low rate of contamination, and the one that did get contaminated was a ceph root ( which I had guessed from the begging would not be sterile).

    Andre

  4. #12

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    Hehe, sounds tricky. Maybe I will get one someday too, but currently, I will rather spend the money on other stuff or more cps [img]http://www.**********.com/iB_html/non-cgi/emoticons/biggrin.gif[/img] . I mean, it's not really the cheapest [img]http://www.**********.com/iB_html/non-cgi/emoticons/laugh.gif[/img] . Zongyi
    What you want to do is illeagle here in Canada.
    Email does not work! Use PM or yangzongyi@hotmail.com instead.

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