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Thread: Byblis liniflora germination....

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    Michael's Avatar
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    PinguiculaMan--when you say a weak bleach solution--what's your formula/recipe?
    Fishing: A Perpetual Series of Occasions for Hope....

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    Moderator Joseph Clemens's Avatar
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    1::10; 1 part bleach to 10 parts water

    -------------------

    I first put the seeds in a tea cup, pour water (hot, but not too hot to touch) enough to cover the seed about 1 inch deep. Let them soak for several hours (this is the first stage of germination -- called imbibition) to initiate the germination process. Then I put a paper towel in a plastic funnel, pour the seed out onto the paper in the funnel so the liquid drains off, then I used a plastic irrigation bottle with a 1::10 bleach solution to wash the seed until no more purple pigment leached from the seed. I then returned the seed to the cup and soaked them again in water to rinse off most of the bleach. Then I sowed them on the surface of moist peat moss and placed them into a ziploc bag under fluorescent lights. They germinated in less than 24 hours.

    -----------

    I discovered this by accident, when I was preparing some seed to sow in vitro I noticed there was purple leaching into the paper towel from the seed, so I continued the bleach rinse until it had all leached out of the seed and into the paper towel. After preparing the seed to sow I then planted half in moist peat moss and the other half in petri dishes of sterile nutrient agar. Wow, was I surprised when they all germinated within 24 hours.



    Joseph Clemens
    Tucson, Arizona, U S A

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    Michael's Avatar
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    Thanks for the ratio--I think even I can handle that--there just might be better living through chemistry! [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_n_32.gif[/img]

    Mike
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    ChronoKiento's Avatar
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    PinguiculaMan, that's amazing!!! I'll have to try that when my byblis gives me some seeds! It's a fairly simple process too! I have one question though, what's an irrigation bottle?

    You should really get these instructions put on the drosera forum as a sticky, I know a lot of people don't know how to germinate byblis correctly and this is a huge breakthrough! Germination in 24 hours! WOW!
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    Question

    this is amazing i new discovery, i wonder if it works on those imposible-to-germinate drosophylum lusticanum seeds?
    [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_k_ani_32.gif[/img]
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    7santiago's Avatar
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    a new discovery, typo, it almost sounds greedy [img]http://www.**********.com/iB_html/non-cgi/emoticons/biggrin.gif[/img] [img]http://www.**********.com/iB_html/non-cgi/emoticons/new/smile_h_32.gif[/img]
    Mens Et Manus

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    chloroplast's Avatar
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    Sounds like a very nice germination technique; thank you. I'll have to try it on a portion of the seed next time I have some.
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    Moderator Joseph Clemens's Avatar
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    Quote Originally Posted by [b
    Quote[/b] ]what's an irrigation bottle?
    Here is one style: http://www.southwestmedical.com/ab15880....ATID=61

    and others:
    http://www.labdepotinc.com/Category_Details~id~370.aspx
    Joseph Clemens
    Tucson, Arizona, U S A

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