Page 10 of 12 FirstFirst ... 6789101112 LastLast
Results 91 to 100 of 113

Thread: Nepenthes in vitro . . .

  1. #91
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Quote Originally Posted by pokie22 View Post
    Those vials look familiar.........
    No doubt, a few of them do. All were freshly replated in new multiplication media last week or so. For whatever reason -- perhaps do to the high amount and variety of PGRs -- these cultures like to "phenolicly bleed" into the media, more so than I would care to admit . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  2. #92
    amphirion's Avatar
    Join Date
    Oct 2009
    Location
    SF Bay Area, US
    Posts
    3,456
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    sweet David!
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  3. #93
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Here is a particularly vigorous culture of Nepenthes edwardsiana, obtained from meristematic tissue; and a new division taken from it, early this morning, on the right . . .

    Nepenthes edwardsiana (Kinabalu: Marai Parai Plateau) 28 August
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  4. #94
    amphirion's Avatar
    Join Date
    Oct 2009
    Location
    SF Bay Area, US
    Posts
    3,456
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    could almost pass for an expensive tillandsia.
    very awesome that you were able to do this via meristematic tissue--i heard the process is difficult because of the cleaning process. will this make cloning other species without the need for seed easier?
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  5. #95
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Quote Originally Posted by amphirion View Post
    could almost pass for an expensive tillandsia.
    very awesome that you were able to do this via meristematic tissue--i heard the process is difficult because of the cleaning process. will this make cloning other species without the need for seed easier?
    Thanks . . .

    It could be replicated; though the real challenge is to carefully excise the desired tissue, using sterile technique, so far as possible. The rationale behind harvesting meristematic tissue is that that tissue is "new" and replicates, in theory, more rapidly than potential viruses and / or endosymbiotic fungi or algae which seem to be the fundamental issue with the use of Nepenthes explants . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #96
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    After a few recent requests, here is a fresh update, of one of the happy cultures:

    Nepenthes edwardsiana (Kinabalu: Marai Parai Plateau) 29 November
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  7. #97
    zesty. BioZest's Avatar
    Join Date
    Mar 2011
    Location
    NE, US
    Posts
    769
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    This is such an amazing thread! Those plants look amazing. How long do you keep these plants in TC? Do you ever lose plants taking them out? Thanks so much for this awesome post.

  8. #98

    Join Date
    Oct 2013
    Posts
    88
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    you have to harden the plants to outside conditions somehow, otherwise you lose them right?

  9. #99
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Quote Originally Posted by BioZest View Post
    This is such an amazing thread! Those plants look amazing. How long do you keep these plants in TC? Do you ever lose plants taking them out? Thanks so much for this awesome post.
    Thanks . . .

    The time spent in a in vitro varies; and the plants in the above photos have already been divided and subdivided on a couple of occasions. The original material, from germinated seed, was "flasked" in 2012; and I have other cultures which predate that . . .

    Quote Originally Posted by Newman View Post
    you have to harden the plants to outside conditions somehow, otherwise you lose them right?
    Yes, the plants go through an establishment phase, where they are kept under high humidity for several weeks and gradually exposed to "harsher" conditions. Some genera are heartier than others; and highland Nepenthes, thankfully, seem to fit that bill . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  10. #100
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,913
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Here is yet another example of seed-grown variation among plants; and, clearly, why particular clones are chosen, over others, during micropropagation. The vials below contained seed, from the same batch, which germinated within days of one another; grew within inches of one another; were exposed to identical lighting and photoperiods; were "plated" in the very same media, etc.

    Those plants on the left have readily multiplied; have been subdivided, while those others seem little more developed since their germination . . .

    Nepenthes villosa (Tambuyukon) 18 June
    Last edited by BigBella; 06-18-2014 at 03:38 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

Page 10 of 12 FirstFirst ... 6789101112 LastLast

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •