Informational! Informational!:  2
Likes Likes:  1
Page 2 of 12 FirstFirst 123456 ... LastLast
Results 11 to 20 of 113

Thread: Nepenthes in vitro . . .

  1. #11
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,957
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Thanks, all . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  2. #12
    Cosmo's Avatar
    Join Date
    Aug 2010
    Posts
    69
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Very well done! They look really promising. I you don't mind answering, for how long and at what concentration did you disinfected the seeds?

  3. #13
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,957
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Quote Originally Posted by Cosmo View Post
    Very well done! They look really promising. I you don't mind answering, for how long and at what concentration did you disinfected the seeds?
    For the last several years, I have only used solutions of PPM (plant preservative mixture) and MS salts to sterilize seed -- particularly Nepenthes, which seem far more susceptible to bleach and / or alcohol damage than most other genera (and is the only consistently-successful method for sterilizing Cephalotus seed that I have found). I alternate between four and eight percent solutions mixed with 1:3 MS salts (just dissolved in distilled water without sucrose, PGRs, or pH adjustment). The seeds, in filter paper envelopes, spend upwards of three to four hours and are plated without rinsing.

    There is a blurb on the ICPS site which goes into some detail about the use of PPM and refutes the popular idea that it inhibits germination:

    http://icps.proboards.com/index.cgi?...ad=5073&page=1
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  4. #14
    instigator thez_yo's Avatar
    Join Date
    Sep 2009
    Location
    San Diego, USA
    Posts
    5,006
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Oooh *golf claps* well done, sir!

  5. #15
    BigBella's Avatar
    Join Date
    Jun 2008
    Location
    SF, CA
    Posts
    2,957
    Mentioned
    1 Post(s)
    Tagged
    0 Thread(s)
    Here is a shot from this morning of a culture shown earlier in this thread. They are seed from Nepenthes macrophylla, sown on 9 September; and they currently seem to be enjoying themselves in a "spa treatment" of 1:4 MS with 1 ml/L BAP . . .

    Nepenthes macrophylla -- 14 November
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #16
    Cardiac Nurse JB_OrchidGuy's Avatar
    Join Date
    Apr 2005
    Location
    SC
    Posts
    3,818
    Mentioned
    2 Post(s)
    Tagged
    0 Thread(s)
    OMG Big Bella I am green with envy. Fantastic work. I am very interested in the processes you use for germination in sterile media. I have a very good sterile tech, but never took chemistry in college for my nursing degree. So mixing the agar plates and getting the right PH is still new to me. I made one attempt at growing orchid seeds, but figured I would wait till I had a better place to work in and could keep the flasks under lights.

    Maybe one day I could pick you brain and get some pointers on the processes used?
    JB
    Friend me on facebook with JB_orchidguy@yahoo.com.
    Growlist Updated 05/08/13

  7. #17
    jimmy uphwiz's Avatar
    Join Date
    Oct 2009
    Location
    ease of blueridge VIRGINIA
    Posts
    1,681
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    wow great work , but your messing up da smart curve here ! LOL

  8. #18
    i dont do pots. amphirion's Avatar
    Join Date
    Oct 2009
    Location
    SF Bay Area, US
    Posts
    3,491
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    very nice David!
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  9. #19
    dashman's Avatar
    Join Date
    Sep 2008
    Location
    Indiana, USA
    Posts
    1,575
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Babies are so cute no matter what Kingdom. Congrats.

  10. #20

    Join Date
    May 2011
    Location
    Adelaide south Australia
    Posts
    99
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    BB great stuff , keep us informed
    please
    J

Page 2 of 12 FirstFirst 123456 ... LastLast

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •