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Thread: Nepenthes in vitro . . .

  1. #33
    BigBella's Avatar
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    Here is another round of seed, getting ready for aseptic germination -- just a few short hours from a FEDEX envelope (a standing project for a good friend of mine with little time on her hands -- something having to do with the care of two teenage girls) . . .

    Nepenthes jacquelinae 30 November


    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  2. #34
    sarracenia lover dionae's Avatar
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    Good luck BigBella! I hope you get good germination rates from the seed.

  3. #35
    instigator thez_yo's Avatar
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    Jeez you just have seeds of everything! When you get AW's Doorman's top 1 and pitopangii into culture, can I have one of each?

  4. #36
    BigBella's Avatar
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    Quote Originally Posted by thez_yo View Post
    Jeez you just have seeds of everything! When you get AW's Doorman's top 1 and pitopangii into culture, can I have one of each?
    For a batch of your cookies and a cup of rocket-fuel coffee, anything you like . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  5. #37
    BigBella's Avatar
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    Here is a vial with a single seedling from this morning, in some experimental media I have made for Nepenthes -- particularly low in ammonium compounds (both nitrates and chlorides), to which they are very sensitive. A full strength batch of Muroshige and Skoog (MS) media, for example, has something on the order of 1650 mg/L of ammonium nitrate; whereas, the mix currently being used has less than ten percent of that amount.

    The first true leaves are just developing as the cotyledons begin to darken . . .

    Nepenthes "sp. A" 1 December


    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

  6. #38
    sarracenia lover dionae's Avatar
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    That culture looks great!

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    very good BB great experiments your doing
    j

  8. #40
    BigBella's Avatar
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    Here is an update on one of the highland Nepenthes species illustrated at the beginning of this post; and I am still getting new germination almost six months out. The most successful seed batches to date were kept under highland conditions from the very start: relatively warm days (as high as 24˚ C) and cold nights (as low as 10˚ C) . . .

    Nepenthes sp. "A" 15 February 2012

    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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