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Thread: Nepenthes in vitro . . .

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    BigBella's Avatar
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    Nepenthes in vitro . . .

    Here are a few shots of some highland Nepenthes seeds within a few days of their aseptic germination in 1:3 Murashige and Skoog (MS) media. At the left, the radicle and hypocotyl are visible, just emergent; and cotyledons can be seen, just unfolding, at the right -- and escaping the seed coat below.

    Nepenthes sp. -- 9 October 2011




    Last edited by BigBella; 10-13-2011 at 12:02 AM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    just your friendly neighborhood INTJ... amphirion's Avatar
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    great work david--how long did it take for the seed to germinate from the time you introduced the seed to the media?

    also i've been wondering...is it possible to induce a callous stage for nepenthes from seed? i know in regular TC methods, one would place the TC flask with plant tissue into a dark area and rotate the flask on some sort of spinner, which would prevent the cells from differentiating and only focus on dividing and absorbing nutrients from the gel... would be great if we can obtain multiple plants out of seed instead of just one.

    thanks for sharing. it's always great to see how the pros do it...

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    cpsammich's Avatar
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    Very cool
    Amp what up wit yo avtar

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    BigBella's Avatar
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    Quote Originally Posted by amphirion View Post
    how long did it take for the seed to germinate from the time you introduced the seed to the media?

    also i've been wondering...is it possible to induce a callous stage for nepenthes from seed? i know in regular TC methods, one would place the TC flask with plant tissue into a dark area and rotate the flask on some sort of spinner, which would prevent the cells from differentiating and only focus on dividing and absorbing nutrients from the gel... would be great if we can obtain multiple plants out of seed instead of just one.
    The bulk of the seed took about twenty-seven days in the various media before germination was seen (courtesy of a magnifying loupe); and, yes, it is possible to induce callus just by tweaking the the various plant growth regulators without the use of either darkness or a spinner. A media containing a large amount of 6-benzylaminopurine (BAP) is usually sufficient to do the job . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Rob's Avatar
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    Can't wait for those EB seeds!

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    Carnivorous plant enthusiast vraev's Avatar
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    lol!! BigB...I sometimes wonder....shouldn't you be drowing in neps and helis by now? lol! Beautiful pic btw...and thanks for sharing. Not often do we see a nep germinating.

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    BigBella's Avatar
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    Here are a few shots of 2011 Nepenthes macrophylla and N. lowii within days of germination. They're certainly nothing to look at for the time being; but it was encouraging, nonetheless, to see that the seed was viable -- and that I knew what I was doing, in an offhand way . . .

    Nepenthes macrophylla -- 18 October



    Nepenthes lowii


    Here is a representative N. macrophylla from the 2009-10 seed batch:

    Last edited by BigBella; 10-18-2011 at 02:55 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    Av8tor1's Avatar
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    ohhhhhhhh me like


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    stretch.... yawn... jbradt's Avatar
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    Awesome, dude. That's really cool.
    Indeed. Most indeededly. Phillip J. Frye


    My growlist.

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    "Planting oblivion,beating reason back..." -[Ven 55.7] Redflytrap's Avatar
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    Quite impressive work David!

    They are germinating very well in your care.
    "How doth my dear morsel, thy mistress?"
    -Shakespere [MM 3.02. 54p]

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