User Tag List

Informational! Informational!:  2
Likes Likes:  1
Page 3 of 10 FirstFirst 1234567 ... LastLast
Results 17 to 24 of 77

Thread: Nepenthes, Cephalotus, Drosera, Heliamphora ...etc, in vitro

  1. #17
    i dont do pots. amphirion's Avatar
    Join Date
    Oct 2009
    Location
    SF Bay Area, US
    Posts
    3,498
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    sweet progress pokie! thanks for sharing.
    parafilm.....fun fun fun fun....
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  2. #18
    pokie22's Avatar
    Join Date
    Sep 2012
    Location
    Bay Area, CA
    Posts
    324
    Mentioned
    0 Post(s)
    Tagged
    1 Thread(s)
    Parafilm was my first friend in lab. I use to wrap it around my fingers all the time. However, it did not bode well when I exhausted the roll and had to explain that I used it for finger wrapping......

    Dry ice is also another favorite. I have fond memories of witch's brew from my elementary school days so I filled all the sinks with dry ice and added water. The entire lab was in smoke - lovely. That, and a couple plugged sinks later + boiling liquid Drano (10M NaOH), and everything was back to normal.

  3. #19
    pokie22's Avatar
    Join Date
    Sep 2012
    Location
    Bay Area, CA
    Posts
    324
    Mentioned
    0 Post(s)
    Tagged
    1 Thread(s)
    The defunct cabbages are about to flower. See BigBella, I have patience. Almost a full circle from seed....

    Pinguicula Lusitanica


  4. #20
    DonH's Avatar
    Join Date
    Apr 2012
    Location
    Southern California
    Posts
    387
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Wow, those things grow fast!

  5. #21
    i dont do pots. amphirion's Avatar
    Join Date
    Oct 2009
    Location
    SF Bay Area, US
    Posts
    3,498
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    i believe P. lusitanica is an annual. so it would make sense that they would flower about now. and joy, no need to sterilize seed!
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  6. #22
    pokie22's Avatar
    Join Date
    Sep 2012
    Location
    Bay Area, CA
    Posts
    324
    Mentioned
    0 Post(s)
    Tagged
    1 Thread(s)
    Comparison of Nepenthes Attenboroughii sprouts in vitro vs. compost





    How do yours look Don?

  7. #23
    DonH's Avatar
    Join Date
    Apr 2012
    Location
    Southern California
    Posts
    387
    Mentioned
    0 Post(s)
    Tagged
    0 Thread(s)
    Your in vitro is further along than mine. Mine has a lot of bruising because these gorilla hands can't handle tweezers when I was replating them into new media with less agar. They also seem to be forming calluses instead of pitchers. I'll try to take picture tomorrow.

    The ones have have in compost are larger with most of them working on their fourth pitchers.

  8. #24
    pokie22's Avatar
    Join Date
    Sep 2012
    Location
    Bay Area, CA
    Posts
    324
    Mentioned
    0 Post(s)
    Tagged
    1 Thread(s)
    Use the loops! Tip the jar/vessel at 45deg angle and use the loop to scoop them out gently. When the sprouts get bigger, the loops can not be used. But at this stage it is still fine. The leaves touching, or submerged under, the media have black spots. Also, there are gaseous bubbles trapped under the plant from respiration. Ah, my hands are not the smallest either, size 7. Grip training does not help matters.

    The compost one is on the 4th pitcher as well. The oldest pitchers just passed away. I moved it to HL conditions yesterday.

Page 3 of 10 FirstFirst 1234567 ... LastLast

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •