All purpose thread for all the cultures I have in the works. Most of my cultures are Nepenthes, but I should have a couple of everything...In the last two months, I have amassed nearly 100 cultures. I have listed the media in cases where it is not clear. Here are a few of the cute sprouts:
Nepenthes Attenboroughii (Jeremiah) 2 month old seedling on 1/4MS+2.5%sucrose+1mg/L BAP, ph5.6
Nepenthes Attenboroughii (Jeremiah)2 month old seedling; compost control
Nepenthes Glabrata putative
Nepenthes Glabrata; compost control putative
Drosera Regia in vitro
Drosera Regia; compost control
Pinguicula Lusitanica on ¼ MS + 1x Vits ph 5.75
Dionaea Fused Fuzzy on ⅓ MS + 1X Vits+ 3% sucrose ph 5.6
Dionaea Sawtooth II on ⅓ MS + 1X Vits+ 3% sucrose ph 5.6
Sarracenia Alabamensis; 24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Sarracenia Jonesii -- red/HV;24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Sarracenia Oreophila;24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Darlingtonia Californica, Siskiyou Co; 42hr 500ppm ga3 soak onto 2/3KC+3%sucrose+0.5mg/L NAA ph5.8
Darlingtonia Californica, Del Norte Co; 42hr 500ppm ga3 soak onto 1/3MS+3%sucrose+1mg/L BAP ph5.6
Darlingtonia Californica, Del Norte Co; 42hr 500ppm ga3 soak onto 2/3KC+3%sucrose+1mg/L NAA ph5.6
In my hands, Darlingtonia California necessitates a ga3 treatment (or stratification) to break dormancy. My controls without ga3 +/- NAA or BAP, are not showing any signs of germination. Also, no cute sprouts on any media with NAA. Instead, they look like those white Chinese steamed buns (callus perhaps..) during the first rise.
Nepenthes Attenboroughii (Jeremiah) 2 month old seedling on 1/4MS+2.5%sucrose+1mg/L BAP, ph5.6
Nepenthes Attenboroughii (Jeremiah)2 month old seedling; compost control
Nepenthes Glabrata putative
Nepenthes Glabrata; compost control putative
Drosera Regia in vitro
Drosera Regia; compost control
Pinguicula Lusitanica on ¼ MS + 1x Vits ph 5.75
Dionaea Fused Fuzzy on ⅓ MS + 1X Vits+ 3% sucrose ph 5.6
Dionaea Sawtooth II on ⅓ MS + 1X Vits+ 3% sucrose ph 5.6
Sarracenia Alabamensis; 24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Sarracenia Jonesii -- red/HV;24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Sarracenia Oreophila;24hr h2O presoak + 42hr 500ppm ga3 soak onto 1/3MS+1xvits+3%sucrose ph5.8
Darlingtonia Californica, Siskiyou Co; 42hr 500ppm ga3 soak onto 2/3KC+3%sucrose+0.5mg/L NAA ph5.8
Darlingtonia Californica, Del Norte Co; 42hr 500ppm ga3 soak onto 1/3MS+3%sucrose+1mg/L BAP ph5.6
Darlingtonia Californica, Del Norte Co; 42hr 500ppm ga3 soak onto 2/3KC+3%sucrose+1mg/L NAA ph5.6
In my hands, Darlingtonia California necessitates a ga3 treatment (or stratification) to break dormancy. My controls without ga3 +/- NAA or BAP, are not showing any signs of germination. Also, no cute sprouts on any media with NAA. Instead, they look like those white Chinese steamed buns (callus perhaps..) during the first rise.