I am looking here to see if anyone has experience with working with villosa and macrophylla tissue samples and being able to get a callus to form for multiplication. Each Nepenthes species seems to have its own preference for how much BAP and MS it likes, so by getting a good idea for both these plants I can be able to find a common ground media that may work well for both. My theory behind this is the genetic similarities to N. villosa and N. macrophylla, since they are so close on a family level that means their genetic structure is similar and therefore their metabolic functions would be similar as well. Using this data will help me in my studies to help clone the tissue of living plants instead of relying solely on seeds in TC.
I know many people say it's near impossible to grow Nepenthes from meristem samples but I believe it can be done and made more easy! I am starting some experiments in my home and my plan is to first properly sterilize the meristem tissue and to then cut the tissue length wise down through the center of the plant to expose the meristem to the media. This i believe has been widely overlooked as a possible cause of why so little samples ever got the chance to grow, the leaves surrounding the meristem acts as a shield which prevents the media from reaching the core of the sample. By exposing this tissue I think it will have a much higher chance of growing and forming a callus.
Ok so lets get some responses in here, media mixes and other advice is greatly appreciated.