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Thread: Looking for female and male nepenthes leaves for sexing experiment

  1. #17
    pebes's Avatar
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    Female
    1. Exo - N. smilesii

    Male
    1. Exo - N. spectabilis
    2.Pebes-N. aristolochioides
    3.Pebes-N. talangensis


    Unknown
    1. Exo - N.hamata, ovata
    2. pebes- N. edwardsiana AW, N. singalana



    thanks

  2. #18
    villosaholic Heli's Avatar
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    Female
    1. Exo - N. smilesii

    Male
    1. Exo - N. spectabilis
    2.Pebes-N. aristolochioides
    3.Pebes-N. talangensis
    4.Heli- N. predator

    Unknown
    1. Exo - N.hamata, ovata
    2. pebes- N. edwardsiana AW, N. singalana
    3. Heli- Burbidgeae sg, edwardsiana sg

    Do we send you a whole leaf or can we send you a piece of a leaf?

  3. #19
    Class 5 Nepenthes hoarder lance's Avatar
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    Female
    1. Exo - N. smilesii
    2. lance - N. Hirsuta X Tiveyi
    3. lance - N. thorelii X (truncata X campunalata)

    Male
    1. Exo - N. spectabilis
    2.Pebes-N. aristolochioides
    3.Pebes-N. talangensis
    4.Heli- N. predator
    5. lance- N. X Aeneas

    Unknown
    1. Exo - N.hamata, ovata
    2. pebes- N. edwardsiana AW, N. singalana
    3. Heli- Burbidgeae sg, edwardsiana sg


    In addition to growing plants, I design and build RC planes powered by Tesla batteries. Check out my progress at www.chargedplanes.com

  4. #20
    pokie22's Avatar
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    Amusing. 2 eddies on the unknown. The impetus for this is actually to determine some UHL sprouts, Edwardsiana being one of them.

    Lance: no unknowns for you?

    Heli: For your own edification and so that you can make a volitional decision - PCR is a method of amplifying a specific DNA sequence by use of a thermostable DNA polymerase and short DNA primers that flank each side of the target sequence. For each cycle, the theoretical amplification is 2^N, where N is the number of cycles. When amplifying from a small amount of DNA, I usually perform 30-40 cycles as a starting point. Theoretically, there are methods of amplifying from less than one molecule. However, I like to exist in the real world and not imaginary. If there is no signal, I usually increase the number of cycles. Now, the more cycles, the more one invites DNA base pair errors. The DNA polymerase has an inherent error rate and the one that is used for amplification of dirty DNA, Taq polymerase, is the most error prone. There are often false positives and false negatives in PCR when performing higher number of cycles. The less starting DNA in the tube = the more cycles will have to be done.

    Now, extrapolating this to real world applications, rapist and serial killers are some times convicted by DNA fingerprinting with PCRs that have undergone 90 cycles. I can vouch that some times I see Jesus in my gels after 90 cycles. Of course, this is only an overly simplistic view of PCR. Everything in life is so much more complicated...

  5. #21
    villosaholic Heli's Avatar
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    so in layman's terms you are trying to find the coding in the DNA that determines the sex of the plant by amplifying it through cycles?

  6. #22

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    technology frightens me.... now where did i leave my tin foil helmet?

  7. #23
    i dont do pots. amphirion's Avatar
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    @pokie: using ePCR or traditional?

    for DNA analysis will you be running the DNA through a next gen sequencer or will you be doing the good ol' DNA gel electrophoresis?
    " You keep using that word. I do not think it means what you think it means." -Inigo Montoya
    +growlist
    +petiolaris drosera going dormant?
    +picture thread

  8. #24
    killerplantsguy's Avatar
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    Female
    1. Exo - N. smilesii
    2. lance - N. Hirsuta X Tiveyi
    3. lance - N. thorelii X (truncata X campunalata)
    4. kpg - maxima (flowered)
    5. kpg - izumiae x ventricosa (flowered)
    Male
    1. Exo - N. spectabilis
    2.Pebes-N. aristolochioides
    3.Pebes-N. talangensis
    4.Heli- N. predator
    5. lance- N. X Aeneas

    Unknown
    1. Exo - N.hamata, ovata
    2. pebes- N. edwardsiana AW, N. singalana
    3. Heli- Burbidgeae sg, edwardsiana sg
    4. kpg - ovata sg
    "You cannot depend on your eyes when your imagination is out of focus" - Mark Twain

    "Don't let it end like this. Tell them I said something." - Pancho Villa, last words.

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