ah. i see. $2400 a pop comes from the cost of a 1 million bp run. 700-800 pb is definitely not 1 million
looking forward to your findings! thanks so much taking this on.
Im looking for a female for sexing experiment
Last edited by pokie22; 03-20-2013 at 11:10 PM.
was a joke indeed
Are you planning to use the primers that were used for cycad or papaya sex determination?
@mikewilder: Which are you referring to? I ordered the one from the paper.
There are some reagents that I am still waiting to arrive. Hopefully, they will come by next week. When it does, I will let everyone know. This way the tissue will be fresh and not sitting in liquid nitrogen.
There are still 3 unknowns available. Ron or Wireman, if you are guys are interested.
Last edited by pokie22; 03-20-2013 at 11:13 PM.
I see, Sudmoon et al. (I thought you were relying on a different paper.) I wish they were less ambiguous about what happens when (if?) you use this primer on smilesii. This will be interesting. If it does turn out to reliably produce a male band in all Nepenthes species, that seems like enough of an assay. Why bother with gel-excision cloning and sequencing? Just to make new primers specifically for that band?
I would like to know what the 750bp band is by running the sequence through BLAST. Of course, the sequence would allow me to design specific primers; 10mers are not good enough. I like nice clean experiments. A ladder is not exact.