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Thread: Looking for female and male nepenthes leaves for sexing experiment

  1. #33
    Maiden's Avatar
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    Im looking for a female for sexing experiment
    Maiden

  2. #34
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    My high school interns are given projects much more complicated than this. Cloning from genomic DNA is straight forward and is usually a part of the building process. Now, some times grocery shopping can get quite complicated, especially if it involves my mother....

    Quote Originally Posted by pebes View Post
    that high? this seems like wipe-up-a-spill type of stuff to me...
    Last edited by pokie22; 03-20-2013 at 11:10 PM.

  3. #35
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    Quote Originally Posted by Maiden View Post
    Im looking for a female for sexing experiment
    Aren't we all.....

  4. #36
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    was a joke indeed
    Maiden

  5. #37

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    Are you planning to use the primers that were used for cycad or papaya sex determination?

  6. #38
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    @mikewilder: Which are you referring to? I ordered the one from the paper.

    There are some reagents that I am still waiting to arrive. Hopefully, they will come by next week. When it does, I will let everyone know. This way the tissue will be fresh and not sitting in liquid nitrogen.

    There are still 3 unknowns available. Ron or Wireman, if you are guys are interested.
    Last edited by pokie22; 03-20-2013 at 11:13 PM.

  7. #39

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    I see, Sudmoon et al. (I thought you were relying on a different paper.) I wish they were less ambiguous about what happens when (if?) you use this primer on smilesii. This will be interesting. If it does turn out to reliably produce a male band in all Nepenthes species, that seems like enough of an assay. Why bother with gel-excision cloning and sequencing? Just to make new primers specifically for that band?

  8. #40
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    I would like to know what the 750bp band is by running the sequence through BLAST. Of course, the sequence would allow me to design specific primers; 10mers are not good enough. I like nice clean experiments. A ladder is not exact.

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