I am soliciting for leaves from known male or female Nepenthes. I promised someone female plants from my Nepenthes sprouts and I intend to keep it. This is a PCR based assay from a 2007 published paper. The techniques utilized are commonplace but the DNA was never sequenced by the researchers. I intend to replicate the protocol, clone, and then sequence the "male specific band" if there is one. I am looking for a small subset of leaves.
Stipulations
1) You must be able to validate the sex of the plant (i.e. have seen it flower. Clones from a TC "male" is not good enough)
2) For every verified leaf, you can send me (1) unknown of your choice which will be in the experimental group
I would prefer growers who live in the bay area. However, I am open to all, as genomic DNA is very stable.
Jen
Stipulations
1) You must be able to validate the sex of the plant (i.e. have seen it flower. Clones from a TC "male" is not good enough)
2) For every verified leaf, you can send me (1) unknown of your choice which will be in the experimental group
I would prefer growers who live in the bay area. However, I am open to all, as genomic DNA is very stable.
Jen