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Thread: 2015 Nepenthes in vitro . . .

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    BigBella's Avatar
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    2015 Nepenthes in vitro . . .

    Here are a few images of multiplying plantlets in a cytokinin-rich experimental media. The challenge with this species, as well as many other highlanders and ultra-highlanders, are the development of roots; or rhizogenesis.

    More often than not, I allow the plants to root ex vitro, as though they were cuttings; it's far cheaper, in terms of labor and materials, and often quicker in the long run . . .

    Nepenthes villosa "Gamma Clone" (Tambuyukon) 18 April





    Nepenthes attenboroughii (Mt. Victoria)



    Last edited by BigBella; 04-19-2015 at 02:18 AM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    This should turn out amazing.

    Out of curiosity, what do you do with the plants once they're rooted?

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    Carnivorous plant enthusiast vraev's Avatar
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    wow. Fantastic job BigB. I hope you eventually manage to open up a shop to sell some of the nice nepenthes species you have managed to produce in vitro. Will be a stellar selection.

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    I Am the Terror Of the Night! NemJones's Avatar
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    thats so cool. Wish i had the money/knowledge to do this kind of stuff

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    BigBella's Avatar
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    Quote Originally Posted by NemJones View Post
    thats so cool. Wish i had the money/knowledge to do this kind of stuff
    Thanks . . .

    I began on a shoestring budget while still at school; made homemade media; scavenged for bottles, etc; and, in terms of knowledge, there is very little pain in cracking a few books . . .
    Last edited by BigBella; 04-22-2015 at 05:54 PM.
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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    villosaholic Heli's Avatar
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    Looks awesome! I look forward to any updates to your coffee thread too.

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    Aristoloingulamata Dexenthes's Avatar
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    Any luck with Sp. "Mt. Mingan"?
    LOOKING FOR: N. (argentii x bicalcarata) x {[(lingulata x edwardsiana) x (naga x hamata)] x [(klossii x undulatifolia) x (aristolochioides x rajah)]} Growlist: http://www.terraforums.com/forums/sh...d.php?t=124586

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    BigBella's Avatar
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    Quote Originally Posted by Dexenthes View Post
    Any luck with Sp. "Mt. Mingan"?
    Sadly, I had repeated contamination issues with that batch. Some lowland and intermediate Nepenthes seed are notoriously difficult to clean effectively and are often riddled with algae and fungi.

    To give you a perspective, the protocol for cleaning highland seeds in a 4% PPM solution (a very effective biocide), is five to eight hours; and lowland species -- Nepenthes ampullaria is a prime example -- are often soaked for an excess of twenty-four or more . . .
    “Sì perché l'autorità dell'opinione di mille nelle scienze non val per una scintilla di ragione di un solo . . ."

    -- Galileo "Biff" Galilei

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