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Thread: Tissue culture update

  1. #1

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    Tissue culture update

    Some of you might remember I posted about 3 weeks back asking for tissue culture advise. Well in any case I just noticed today that I had successful invitro germination of N.alata. D.regia has also begun to sprout.


    Andre

  2. #2

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    Great news Andre.

    Care to share more information on what and how you achived this?

    Got any photos?

    Aaron.

  3. #3

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    I am by no means a TC expert, actually this is the first attempt so I still have allot to learn and plenty can still go wrong but here is what I tried:

    Seed bought from Triffid nurseries in the UK.
    TC kit bought from http://www.kitchenculturekit.com/Index.htm


    For both seeds I tried sterilizing the seed in a 10% bleach solution mixed with a couple drops of sunlight in it for ten minutes.
    I followed this with a dunk into hydrogen peroxide and finally sterilized water to rinse it off.

    For the nepenthes media I am trying MS solution diluted to half strength . I also added 1ml BAP and 1 ml PPM.
    For the Drosera same mix minus the BAP.

    I do not have any pictures yet but if all keeps going well I will take some in a few days and post.

  4. #4

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    That is amazing. Well done!

    Cheers,

    Joe

  5. #5

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    Great for you! I hope all goes well with it and you get to be real good with t\c.
    [img]http://home.**********.com/users/pondboy/Neps/Neps%20sig..JPG[/img]

  6. #6
    HellzDungeon's Avatar
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    nice!!!
    i was looking at that kit to, seeing as my past home made T/C experiment failed...
    is it a good kit? im guessing it is hearing about your results with it, and im am planning on buying one as well
    also, which kit did you buy?
    thanks for your help in advanced,
    Hellz
    Nike SB is Bananas

  7. #7

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    MS? BAP?
    I know PPM though, it's "particules per million" and reprentsents the number of mollecules of said substance in a million mollecules that compose a mix.
    If the dragon is bigger than his treasure, it's not worth the effort.

    My Growlist

  8. #8

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    HellzDungeon, I got the All-Purpose Kitchen Culture Kit ("Five Year Special"), because I have a long list of plants I wish to try TC. This kit comes with allot more product. Also you can tell Carol (the vendor) which mediums you want her to send you in it.

    PPM = Plant Preservative Mixture
    BAP = Benzylaminopurine
    MS = Murashige and Skoog plant growth medium with vitamins and inositol

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