There are two general approaches to tissue culturing (assuming you're a hobbiest). The first being the careful way involving time & effort in preparation. The second being simply jumping into it reading a few threads or maybe a tutorial on the subject. If the second approach is chosen, you'll most likely end up researching after numerous failures & questions or quitting b/c lack of foundation. Either way, you may as well read read & read b/c you will not start mass producing plants overnight. By the way, I'm not at all a professional as some may be. I learned about TC with much research as a kid & also learned about it in college, but I'm simply a home TC hobbiest of many years experience who is still learning.