Make Shift Tissue Culture?

[Vbkid]

So for a while now I've had a strong desire to get started in tissue culture, both for the plant propagation and because the entire process seems awesome. The limiting factor, as a college student, has been money and I've pretty much resigned myself to waiting until I finish up in the fall. However, being on an engineering campus, I've been thinking it might be possible to gather a lot of the supplies from various departments.

So a quick question, what would people recommend be the necessary supplies i might need to all collect? to be clear, I will not be stealing these, but will certainly ask various professors if they might have extra supplies lying around.

Thanks for the help,
Kyle

 

[MICKEY]

glove box or hood ( fan clear, tank, gloves, uv lights)

 

[gill_za]

I'm sure you have seen this link
http://www.flytrapcare.com/tissue-culture-basics.html

there is also bunch of helpful videos that explain the process in details on youtube.

if you want to make your own media.. well it is a multi-component mixture so perhaps it would be easier to just buy it premixed.
I work in the lab and was thinking about mixing it myself but decided against it.


But you might want to wait and see what people who actually DID TC will say about it.

 

[MICKEY]

only pays to mix your own if your trying to find a better mixture or if your on the clock and someone is paying you while your mixing there compounds

 

[Vbkid]

I actually have full access to a top notch hood, and from reading the link (Thanks Gill!) it seems the things I don't have have access too will be:
Baby food jars (easy enough)
Media (any recommendations where/what compound to buy? It will be for Nepenthes seed at first)
Phyto Caps (where to buy?)
Pressure cooker. Is this the only way to sanitize? Does anyone else have a different method?
Thanks again for all the input!

 

[gill_za]

Media (any recommendations where/what compound to buy? It will be for Nepenthes seed at first)

http://www.hometissueculture.org/

they sell kits for beginners tell them that it is for CP's. As far as adjusting the media for neps, you'll have to look around here. I'm pulling this info from my old saved searches on here and google:

Perhaps some of this info will be helpfull (it is a copy of my google docs TC related notes so far)

!!!!!!!!!!!!!!!!!start copy/paste!!!!!!!!!!!!!!
Tissue Culture General info


CP Tissue Culture information
http://www.omnisterra.com/botany/cp/slides/tc/tc.htm
http://www.kitchenculturekit.com/index.htm
http://hometissueculture.org/catalogkits.htm
http://www.terraforums.com/forums/showthread.php?t=128027
http://www.flytrapcare.com/tissue-culture-basics.html
http://icps.proboards.com/index.cgi?board=tissue
http://www.terraforums.com/forums/showthread.php?t=128978&highlight=tissue+culture
http://www.terraforums.com/forums/showthread.php?t=128742&highlight=tissue+culture


Media
http://www.flytrapcare.com/phpBB3/r...red-media-w-other-members-t13038.html#p121640
http://www.youtube.com/watch?v=i8BYAX8JJpM


Sterilization

Bleachless Sterilization
nepenthes tissue culture thread
http://icps.proboards.com/index.cgi?action=display&board=tissue&thread=3147&page=1#20122

Sterilization with Bleach etc

http://www.flytrapcare.com/phpBB3/requesting-to-compare-prepared-media-w-other-members-t13038.html


!!!!!!!!!!!!!!!!!end copy/paste!!!!!!!!!!!!!!

 

[Vbkid]

Found this, looks like a great starting point.
http://icps.proboards.com/index.cgi?board=tissue&action=print&thread=3147

 

[BigBella]

I agree with an earlier post and would also suggest Matt's site, www.flytrapcare.com, where he offers an ongoing forum on tissue culture -- under GROWING ENVIRONMENTS, TISSUE CULTURE AND OTHER PROPAGATION, TOOLS AND TECHNIQUES -- and a clear overview of what is necessary for success . . .

 

[Vbkid]

Thanks Bella, was hoping you might chime in, I've always been amazed at your great successes (coupled with you wonderful pictures!)

 

[BigBella]

Thanks Bella, was hoping you might chime in, I've always been amazed at your great successes (coupled with you wonderful pictures!)

Thanks for the kind words; and pay special attention, early on, to getting the pH of your media correctly adjusted; and that can often be species-specific. Also, forget about using any pH strips (even the expensive variety can be off "by miles") and only use a calibrated meter . . .