Micropropigation from seed help

[jon]

Ok, I got my pressure cooker, I know how to do micropropagation from plant leaves, no I need to find out how to do it with CP seeds.

Do I starelize the seed and just throw it on a petri dish and watch it grow?

Do anyone have advice, etc?

OH!!! LOOK! NEW SMILIES! I've got to use them...let see.

Clockwork Orange!  

Ugh...American Idol....  

WASSS UPPPPP!!!!!!    

ok, now that that's out of my system...

 

[Spectabilis73]

Sterilising is very hard. You might want to stratify first. Here is the protocol I will be using:

Rinse in sterile R/O water (you gotta pressure cook it), room temperature.
Rinse in 10% bleach
Rinse in sterile R/O water, room temperature
Soak in 10% bleach for 5 minutes
Soak in 70% alchohol for 2-5 minutes
Rinse in 5% bleach, Some prefer not to wash the bleach residue off, as it helps keep dampoff away, etc

There is a gradual PPM breakdown once you use the medium. Though the rate is
variable and depends on the number of ex-plants per given volume of medium.
Therefore there is a chance that after long period of culture infiltrated
microbial spores will manage to germinate. However this is more likely to
happen in solid medium.

 

[jon]

What does R/O mean?

 

[superimposedhope]

Reverse Osmosis, I think he means

Joe

 

[jon]

OH! ok, got it!