I'm curious too. Do you see any benefit of doing so for TC?
I'm never able to get 100% sterilisation of Nep seeds, always have some fungus in at least a few seeds. Does removing the seed coat allow a better treatment?
Two of my Neps are flowering for the first time, and looking at the flower I expect both of them to be male (lucky me...). These are pure mudblood, so I don't expect a lot of interest.
Still if you have a female plant without any specific value flowering (I mean if you want to cross it...
1.heffely92-I would like some
2. Grey Moss - I'll throw my hat in. Thanks for the great generosity!
3. charlie - That's very generous! Thanks for the giveaway!
4. Sashoke - Thank you for the opportunity!
6. Thatonechick thanks so much!
7. Raistlarn -. Thank you for the giveaway
8. emc2 -...
I was where you are a few years ago, it's definitively interesting to learn, but takes a lot of time!
I will MP you my pipeline, mostly using SLURM scheduler commands and bash to launch the actual tools.
What software do you use with the minion, can you use an OLC one as you end up with long reads?
I unfortunately have no experience with genomes, only do exomes and transcriptomes, but I can always have a look if I can run it on our HPC cluster.
Was more thinking in shipping a piece of leaf or better a tube of DNA if you can still perform the extraction locally.
Else, yes, totally agree, these are different use case, I guess a better comparison, for costs, would be with a 10x genomics run. I'm actually quite interested into the sample...
I would be interested on your feedback and you protocols over the nanopore sequencing.
Send me a MP if you don't mind sharing.
Depending the output, coverage, etc it seems quite a steep price by sample. The device is cheap, but then with kit and flowcell it seems each sample will run around...